Resistance to southern rice black-streaked dwarf locus qsrbsdv9 and its molecular marker method
A black-streaked dwarf disease and molecular marker technology, which is applied in the field of plant molecular genetics, can solve the problems of unrelated southern rice black-streaked dwarf disease resistance gene/QTL mapping and disease resistance breeding reports.
Active Publication Date: 2021-09-10
广西壮族自治区农业科学院水稻研究所 +2
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Problems solved by technology
However, there are few reports on the genetic characteristics of resistance to black-streaked dwarf disease in southern rice, let alone the mapping of resistance genes / QTLs and breeding for resistance to black-streaked dwarf disease in southern rice.
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Embodiment 1
[0034] Materials and Methods
[0035] step 1 : Construction of Segregating Populations for Genetic Analysis
[0036] Using the imported new material D4 resistant to southern rice black-streaked dwarf disease as the male parent, and the southern rice black-streaked dwarf variety Guanghui 998 as the female parent, F 1 F 2 group. Then self-cross one generation to obtain the derived population F 2：3 Families for identification of resistance to black-streaked dwarf disease of southern rice.
[0037] step 2 : Identification of resistance to southern rice black-streaked dwarf disease in rice using indoor inoculation method
[0038]The healthy young white-backed planthoppers were transferred to diseased rice plants for 2-3 days, and then transferred to healthy TN1 rice seedlings for 10-12 days to pass the cycle period and then used as inoculation transmission media. The inoculation was carried out in the artificial climate room of the Rice Institute of Guangxi Academy of Agri...
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The invention belongs to the field of plant molecular genetics, and relates to a locus qSRBSDV9 resistant to southern rice black-streaked dwarf disease and a molecular marker method thereof. Resistance to southern rice black-streaked dwarf locus qSRBSDV9 and its molecular marker method, F 2 Association analysis and genetic linkage analysis were performed on the population, and the southern rice black-streaked dwarf locus qSRBSDV9 was obtained, which was located between the molecular markers Indel32-Indel35, and the molecular marker Indel32 primer, when qSRBSDV9 existed, the amplified band was a 111bp band; Mark the Indel35 primer, and the amplified band is a 147bp band when qSRBSDV9 exists. The present invention can detect whether the resistant material D4 and its derivative varieties (lines) contain the southern rice black-streaked dwarf gene locus through the molecular marker of the southern rice black-streaked dwarf gene, and can predict its southern rice black-streaked dwarf gene locus. The resistance level of stripe dwarf disease can be improved greatly, and the selection efficiency of rice resistant to black-streaked dwarf disease of southern rice can be greatly improved.
Description
technical field [0001] The invention belongs to the field of plant molecular genetics and relates to loci resistant to southern rice black-streaked dwarf disease qSRBSDV9 and its molecular labeling method. Background technique [0002] Rice is one of the most important food crops in the world, and more than half of the world's population uses rice as the main food. Since the black streak dwarf of southern rice was first discovered in 2001, the disease has spread rapidly in just a few years, causing great losses to the vast rice areas in southern my country and northern Vietnam. Different from common rice black-streaked dwarf disease, southern rice black-streaked dwarf disease is a rice virus disease mainly transmitted by white-backed planthopper, and its pathogenic virus is Southern rice black-streaked dwarf virus (Southern rice black- streaked dwarf virus (SRBSDV for short) is a new species of group 2 of the genus Fijivirus of the Reoviridae family. At present, there is ...
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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 农保选李丹婷夏秀忠杨行海张宗琼曾宇邓国富罗群昌莊洁
Owner 广西壮族自治区农业科学院水稻研究所