Antigen retrieval apparatus and method for tissue section

A technology for tissue sectioning and repairing methods, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of large damage to tissue sections, poor staining effect, and inappropriate de-staining and counterstaining for multiple times, so as to reduce damage and achieve obvious effects. , reduce the effect of turbulent impact

Active Publication Date: 2018-12-04
SHENZHEN UNIV +1
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  • Abstract
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Problems solved by technology

The staining effect of microwave heating antigen retrieval method and special steamer antigen retrieval method is not as good as that of high-pressure water bath antige...

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  • Antigen retrieval apparatus and method for tissue section
  • Antigen retrieval apparatus and method for tissue section
  • Antigen retrieval apparatus and method for tissue section

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Embodiment Construction

[0038] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0039] see Figure 1-Figure 3 , the first embodiment of the present invention proposes a tissue section antigen retrieval device 100 . The tissue section antigen retrieval device 100 includes a first glass slide 110 for fixing the tissue section 112; a second slide 120 arranged at intervals towards the tissue section 112 and the first slide 110; A spacer layer 130 between the glass slide 110 and the second glass slide 120 , the spacer layer 130 is spaced from the tissue section 112 by a certain distance. The first slide 110 , the second slide 120 and the spacer layer 130 together define a containment space with a water seepage structure. In a preferred embodiment, the tissue section 112 is a paraffin-embedded tissue section after dewaxing treatme...

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Abstract

The invention relates to an antigen retrieval apparatus for a tissue section. The antigen retrieval apparatus comprises a first slide for fixing a tissue section, a second slide oriented toward the tissue section and spaced apart from the first slide, and a spacing layer surrounding the tissue section and arranged between the first slide and the second slide, wherein the first slide, the second slide and the spacing layer define accommodation space having a water seepage structure together. The invention also provides an antigen retrieval method for the tissue section. The antigen retrieval apparatus and method for the tissue section in the invention can reduce damage to the tissue section during antigen retrieval.

Description

technical field [0001] The invention relates to a tissue section antigen retrieval device and a tissue section antigen retrieval method, in particular to a tissue section antigen retrieval device and a tissue section antigen retrieval method used in immunohistochemical antibody staining. Background technique [0002] At present, immunohistochemical method is a method that has been routinely used in clinical pathological examination and experimental pathology. There are many types and quantities of antibodies used in immunohistochemistry, and different antibodies have different requirements for antigen retrieval on pathological sections. Only after the antigens in the tissue sections are thoroughly exposed, can most antibodies effectively bind to the antigens and produce specific immunostaining, such as ER, PCNA, etc. At present, the methods of tissue antigen retrieval (antigen retrieval; AR) mainly adopt heat restoration, including: microwave heating antigen retrieval metho...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N33/531
CPCG01N1/30G01N33/531
Inventor 王筠盛司潼李延鹏胡章立谭辉彪朱民
Owner SHENZHEN UNIV
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