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Application of rehmanthin c and salvianolic acid a in promoting the proliferation of cultured bone marrow mesenchymal stem cells and inhibiting replicative senescence

A bone marrow mesenchymal and stem cell proliferation technology, applied in the field of medicine, can solve the unreported problems of stem cell proliferation and replicative aging, and achieve the effects of reducing immune rejection, promoting proliferation, and inhibiting replicative aging

Active Publication Date: 2021-11-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, the effects of rehmanthin C and salvianolic acid A on stem cell proliferation and replicative senescence have not been reported.

Method used

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  • Application of rehmanthin c and salvianolic acid a in promoting the proliferation of cultured bone marrow mesenchymal stem cells and inhibiting replicative senescence
  • Application of rehmanthin c and salvianolic acid a in promoting the proliferation of cultured bone marrow mesenchymal stem cells and inhibiting replicative senescence
  • Application of rehmanthin c and salvianolic acid a in promoting the proliferation of cultured bone marrow mesenchymal stem cells and inhibiting replicative senescence

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Embodiment Construction

[0034] The present invention will be further described below in conjunction with embodiment.

[0035] 1. Material method:

[0036] 1.1 Reagent DMEM low-sugar medium (Gibco BRL, U.S.); fetal bovine serum (FBS, Gibco BRL, U.S.); 0.25wt.% trypsin (containing 0.02wt.% EDTA, Gibco BRL, U.S.); penicillin-streptomycin DNA solution (Gibco BRL, USA); Cell Counting Kit-8 (Shanghai Beyond Biotechnology Co., Ltd., China); Cell Senescence β-Galactosidase Staining Kit (Shanghai Beyotime Biotechnology Co., Ltd., China);

[0037] 1.2 Instruments Electronic balance (AL204, Mettler Toledo, Switzerland); automatic microplate reader (ELX800, BioTekInstruments Inc., USA); carbon dioxide cell incubator (HF90, Health Force, China); biological safety cabinet (Type A2, Beijing East Lianhaer Instrument Manufacturing Co., Ltd., China); ultrapure water instrument (Milli-Q, Millipore Co., the United States);

[0038]1.3 Isolation and culture of BMSCs 3-week-old male SD rats were killed by cervical dislo...

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Abstract

The invention discloses the application of rehinoside C and salvianolic acid A in promoting the proliferation of bone marrow mesenchymal stem cells cultured in vitro and inhibiting replicative senescence. The rehinoside C and salvianolic acid A coexist. Rehmanthin C and salvianolic acid A can promote the proliferation of bone marrow mesenchymal stem cells cultured in vitro and inhibit replicative senescence, providing enough stem cells for clinical treatment. Among them, rehinoside C and salvianolic acid A are commercially available high-purity rehinoside C and salvianolic acid A (content ≥ 98%), and can also be prepared according to conventional methods. Its advantages are: rehmanthin C and salvianolic acid A can promote the proliferation of bone marrow mesenchymal stem cells and inhibit replicative senescence. Stem cell therapy provides enough cells while reducing the body's immune rejection.

Description

[0001] Technical field [0002] The invention belongs to the technical field of medicine, and specifically relates to the application of rehmanthin C and salvianolic acid A in promoting proliferation of bone marrow mesenchymal stem cells cultured in vitro and inhibiting replicative senescence. Background technique [0003] Bone marrow mesenchymal stem cells, as a kind of cell-based medicine, have broad application prospects in diseases such as bone defect, cartilage defect, liver fibrosis, myocardial infarction, pulmonary fibrosis and diabetes. However, in the commercial production process, it is often difficult to expand the production scale of bone marrow mesenchymal stem cells. Among them, the slow proliferation of bone marrow mesenchymal stem cells, aging during the replication process, resulting in the loss of stemness are the two main reasons that limit the expansion of its production scale and hinder the application and transformation of stem cells. [0004] Bone marro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N2501/999
Inventor 彭丽华许学寒欧阳宏伟徐恩峤
Owner ZHEJIANG UNIV