Diagnostic marker of rifampicin resistance of mycobacterium tuberculosis and application thereof

Abstract

The invention discloses a diagnostic marker of rifampicin resistance of mycobacterium tuberculosis. The diagnostic marker is composed of Rv0840c, Rv2386c and Rv1130. When the expression levels of Rv0840c, Rv2386c and Rv1130 are higher than the normal expression level, it is judged that mycobacterium tuberculosis is resistant to rifampicin. The invention discloses the diagnostic marker of rifampicin resistance of mycobacterium tuberculosis to realize rapid clinical detection of rifampicin resistance of mycobacterium tuberculosis and provides a potential target for treating rifampicin resistance.

Description

technical field [0001] The invention relates to a rifampicin resistance diagnostic marker of Mycobacterium tuberculosis and its application. Background technique [0002] Drug-resistant tuberculosis is a major problem in the global tuberculosis prevention and control work, especially multi-drug-resistant tuberculosis and extensively drug-resistant tuberculosis seriously threaten human health. The 2013 report showed that although the number of new tuberculosis patients decreased, the diagnosis and treatment of multi-drug-resistant tuberculosis Far from reaching the expected goal. Timely and effective monitoring of drug-resistant tuberculosis is the key to tuberculosis prevention and control. At present, the diagnosis of drug-resistant tuberculosis in my country's tuberculosis laboratories includes traditional ratio method drug susceptibility, BACTEC MGIT 960 system, and rapid diagnostic methods based on molecular biology. [0003] The existing techniques for detecting isonia...

AI Technical Summary

Problems solved by technology

The disadvantages of the prior art mainly include: it takes a long time, and it needs to be established on the basis of separation and culture for drug sensitivity detection, which takes 4-8 weeks for separation and culture, and on this basis, the follow-up traditional solid ratio method (4-8 weeks) Or the time of liquid drug sensitivity (1-2 weeks); limited space, both culture methods require a relatively large incubator, and a fixed culture room is required to ensure biological safety; drug resistance targets are limited, and emerging molecular biology The rapid diagnosis technology based on the method greatly shortens the diagnostic cycle within 1-2 hours, but limited by the current research level, the current optimal Xpert MTB / RIF rifampicin resistance detection target is limited to the mutation site of the ropB gene; In clinical cases, Xpert MTB / RIF reports rifampicin resistance but is clinically sensitive to rifampicin, suggesting that rifampicin resistance may have mutations in other gene loci

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