Rifampicin tolerance diagnosis marker for mycobacterium tuberculosis and application of rifampicin tolerance diagnosis marker

Abstract

The invention discloses a rifampicin tolerance diagnosis marker for mycobacterium tuberculosis. The rifampicin tolerance diagnosis marker comprises Rv2243 and Rv0644c; when the expression quantity ofthe Rv2243 and the Rv0644c is lower than the normal expression quantity, the condition that the mycobacterium tuberculosis is tolerant to rifampicin is determined. By the rifampicin tolerance diagnosis marker for the mycobacterium tuberculosis, rapid clinical detection of the rifampicin tolerance to the mycobacterium tuberculosis can be realized, and potential target spots for rifampicin tolerancetreatment are provided.

Description

technical field [0001] The invention relates to a diagnostic marker for rifampicin drug resistance of Mycobacterium tuberculosis and its application. Background technique [0002] Drug-resistant tuberculosis is a major problem in the global tuberculosis prevention and control work, especially multi-drug-resistant tuberculosis and extensively drug-resistant tuberculosis seriously threaten human health. The 2013 report showed that although the number of new tuberculosis patients decreased, the diagnosis and treatment of multi-drug-resistant tuberculosis Far from reaching the expected goal. Timely and effective monitoring of drug-resistant tuberculosis is the key to tuberculosis prevention and control. At present, the diagnosis of drug-resistant tuberculosis in my country's tuberculosis laboratories includes traditional ratio method drug susceptibility, BACTEC MGIT 960 system, and rapid diagnostic methods based on molecular biology. [0003] The existing techniques for detecti...

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Problems solved by technology

The disadvantages of the prior art mainly include: it takes a long time, and it needs to be established on the basis of separation and culture for drug sensitivity detection, which takes 4-8 weeks for separation and culture, and on this basis, the follow-up traditional solid ratio method (4-8 weeks) Or the time of liquid drug sensitivity (1-2 weeks); limited space, both culture methods require a relatively large incubator, and a fixed culture room is required to ensure biological safety; drug resistance targets are limited, and emerging molecular biology The rapid diagnosis technology based on the method greatly shortens the diagnostic cycle within 1-2 hours, but limited by the current research level, the current optimal Xpert MTB / RIF rifampicin resistance detection target is limited to the mutation site of the ropB gene; In clinical cases, Xpert MTB / RIF reports rifampicin resistance but is clinically sensitive to rifampicin, suggesting that rifampicin resistance may have mutations in other gene loci

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