Reagent kit for detecting porcine delta coronaviruses and acute diarrhea syndrome coronaviruses

Abstract

The invention provides a reagent kit for detecting porcine delta coronaviruses and acute diarrhea syndrome coronaviruses porcine delta coronavirus. According to a primer and probe (SEQ ID NO: 1-6) fordouble real-time fluorescent quantitative PCR detection of the porcine delta coronaviruses and the porcine acute diarrhea syndrome coronaviruses, and a double real-time fluorescent quantitative PCR detection method established based on the primer and the probe, and rapid identification and detection of the two viruses in one reaction can be realized at the same time. The reagent kit can completedetection within 2 hours, has the advantages of quickness, specificity, sensitivity, high flux and the like, and can meet the requirements of quickly identifying and detecting the porcine delta coronaviruses and the porcine acute diarrhea syndrome coronaviruses on a large scale.

Description

technical field [0001] The invention relates to the technical field of nucleic acid detection, in particular to a detection kit for porcine delta coronavirus and acute diarrhea syndrome coronavirus. Background technique [0002] Coronaviruses (CoV) belong to the Coronaviridae subfamily of the Coronaviridae family and are a single-stranded positive-sense RNA virus with an envelope, which has the largest genome sequence length among known RNA viruses. Currently, there are five known porcine coronaviruses, including porcine epidemic diarrhea virus (porcineepidemicdiarrhea virus, PEDV), porcine transmissible gastroenteritis virus (transmissible gastroenteritisvirus, TGEV), porcine respiratory coronavirus (porcine respiratory coronavirus, PRCV) , porcine hemagglutinating encephalomyelitis virus (PHEV), porcine deltacoronavirus (PDCoV) and porcine acute diarrhea syndrome coronavirus (Swine Acute Diarrhea Syndrome coronavirus, SADS-CoV). Among them, PEDV, TGEV, PDCoV, and SADS-CoV...

AI Technical Summary

Problems solved by technology

The symptoms of pigs infected with PDCoV diarrhea are very similar to those of PEDV and transmissible gastroenteritis virus (TGEV). At the same time, there is also a phenomenon of mixed infection of the three viruses clinically. Therefore, it is difficult to effectively distinguish these viral pathogens clinically.

[0004] The symptoms caused by SADS-CoV include pig acute diarrhea, weight loss, and death in severe cases, which are very similar to the diarrhea symptoms caused by other porcine enteroviruses, and it is difficult to distinguish them clinically and histopathologically, which brings new challenges to the diagnosis of the disease. It is difficult and needs to be diagnosed with laboratory diagnostic techniques, while traditional detection methods are time-consuming and laborious, and the operation methods are cumbersome, which is not conducive to the rapid diagnosis and treatment of diseases

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