Aminosalicylic acid-resistant diagnostic marker of mycobacterium tuberculosis and application thereof

Abstract

The invention discloses an aminosalicylic acid resistance diagnostic marker of Mycobacterium tuberculosis, Rv3890c, Rv2002, Rv1886c, Rv3824c, Rv3825c. When the expression levels of Rv3890c, Rv2002, Rv1886c, Rv3824c, Rv3825c were lower than the normal expression levels, it was judged that Mycobacterium tuberculosis was resistant to aminosalicylic acid. The invention discloses a mycobacterium tuberculosis drug resistance diagnostic marker capable of realizing rapid clinical detection of mycobacterium tuberculosis drug resistance to aminosalicylic acid, and provides a potential target for treating drug resistance to aminosalicylic acid.

Description

technical field [0001] The invention relates to a diagnostic marker for drug resistance to aminosalicylic acid of Mycobacterium tuberculosis and its application. Background technique [0002] Drug-resistant tuberculosis is a major problem in the global tuberculosis prevention and control work, especially multi-drug-resistant tuberculosis and extensively drug-resistant tuberculosis seriously threaten human health. The 2013 report showed that although the number of new tuberculosis patients decreased, the diagnosis and treatment of multi-drug-resistant tuberculosis Far from reaching the expected goal. Timely and effective monitoring of drug-resistant tuberculosis is the key to tuberculosis prevention and control. At present, the diagnosis of drug-resistant tuberculosis in my country's tuberculosis laboratories includes traditional ratio method drug susceptibility, BACTEC MGIT 960 system, and rapid diagnostic methods based on molecular biology. [0003] The existing technology...

AI Technical Summary

Problems solved by technology

The disadvantages of the prior art mainly include: (1) It takes a long time, and it needs to be established on the basis of separation and culture to carry out drug sensitivity detection. The separation and culture need 4-8 weeks, and on this basis, follow-up traditional solid ratio method (4-8 weeks ) or liquid drug susceptibility (1-2 weeks); ⑵ limited space, both culture methods require a relatively large incubator, and a fixed culture room is required to ensure biosafety; 3 relative to rifampicin and isoniazid However, there are no molecular detection targets and related mature molecular diagnostic products for aminosalicylic acid resistance.

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