Primer pair for dual-LAMP simultaneous detection of shigella and listeria monocytogenes, reagent, kit and method

Abstract

The invention discloses a primer pair for dual-LAMP simultaneous detection of shigella and listeria monocytogenes, a reagent, a kit and a method. Through the unique primer design, the simultaneous detection of shigella and listeria monocytogenes can be realized; the primer pair has the advantages of short detection period, high detection speed, and the like; a dual-LAMP detection method adopted bythe invention is capable of qualitatively analyzing if the meat products contain shigella and listeria monocytogenes, taking the advantage of LAMP detection technology, further perfecting a detectionsystem for food-borne pathogenic bacteria in the meat products and promoting the protection for consumer benefits.

Description

technical field [0001] The invention discloses the field of loop-mediated isothermal amplification technology, and particularly relates to a primer set, reagent, kit and method for dual LAMP simultaneous detection of Shigella and Listeria monocytogenes. Background technique [0002] At present, the techniques used to detect pathogenic bacteria in food at home and abroad mainly include traditional national standard detection methods, PCR methods, fluorescent quantitative PCR and multiplex PCR detection methods. [0003] With the development of the market economy, the production and consumption of meat products have maintained a continuous development trend, and the demand for imported and exported meat products has continued to increase. However, Shigella and Listeria monocytogenes commonly found in some meat products Tertiary bacteria adopts the traditional culture method, and each pathogenic bacteria needs to apply an independent detection method, which not only requires a ...

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Problems solved by technology

[0004] In view of this, the present invention discloses a dual LAMP primer set, reagent, kit and method for simultaneous detection of Shigella and Listeria monocytogenes, to at least solve the problem that the previous detection methods cannot achieve the above bacterial species Simultaneous detection, long detection cycle, high misjudgment rate, etc.

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