Device and method for liquid transfer type nucleic acid extraction

Abstract

The invention provides a device and a method for liquid transfer type nucleic acid extraction. According to the invention, a through rail is connected with and arranged at the central part of a frame;a beam arm is connected with and arranged on the through rail; a clamping mechanism is connected with and arranged at one side of the beam arm; a sample addition mechanism is connected with the otherside of the beam arm; the clamping mechanism moves along the beam arm in a Y direction; the clamping mechanism itself moves in a Z direction; the sample addition mechanism moves along the beam arm ina Y direction; the sample addition mechanism itself moves in a Z direction; the interiors of each reagent carrying support and each PCR refrigeration mechanism are respectively provided with semiconductor refrigeration sheets; each deep-hole plate vibration heating mechanism comprises an vibration motor which is connected with a vibration eccentric wheel; the upper end of the vibration eccentricwheel is connected with a heating aluminum block; the upper end of the heating aluminum block is connected with a heating top plate; the lower end of the heating aluminum block is connected with shockdampers; and the outer sides of the heating aluminum block and the heating top plate are connected with vibration heating baffle plates. The device and the method provided by the invention realize continuous and automatic sample addition, reagent addition, transferring, incubation and extraction, can refrigerate a reagent or an extracted product and disinfect a used sucker, and have high extraction efficiency.

Description

technical field [0001] The invention relates to a liquid transfer type nucleic acid extraction device and method, and relates to the technical field of nucleic acid extraction. Background technique [0002] Since the birth of Watson-Crick's DNA double helix model, biology has entered a new era. In the field of biology, whether it is the detection of pathogenic microorganisms, species identification, or the origin of species, diversity assessment and their relatives The research on relationships, system evolution, etc., all need to separate and purify nucleic acid first, so the extraction of DNA has become the basis for scientific research in all disciplines in the fields of biomedicine and even agriculture, forestry, animal husbandry and fishery. Whether high-quality nucleic acid can be extracted is the key to nucleic acid molecular biology experiments, and the sensitivity and specificity of the extraction method will also directly affect the success or failure of subsequent...

AI Technical Summary

Problems solved by technology

The existing nucleic acid extraction technology solutions cannot realize automatic and continuous sample addition, reagent addition, incubation, and extraction processes, and cannot realize the disinfection of tips. Nucleic acid extraction usually needs to be performed between multiple separate devices, and the overall extraction efficiency Low, the reagent or the extracted product cannot be refrigerated during the extraction process, and the extracted DNA is easily broken, which affects the test effect

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