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Detection method and detection kit for EML4-ALK fusion gene

A technology of EML4-ALK-1 and fusion genes, applied in the field of biomedicine, can solve the problems of lack of EML4-ALK gene detection methods, obstacles to patient treatment and clinical research

Inactive Publication Date: 2018-12-14
为康(苏州)基因科技有限公司
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Problems solved by technology

[0004] However, due to the lack of accurate and efficient detection methods for EML4-ALK gene fusion, it hinders the treatment of patients and clinical research

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  • Detection method and detection kit for EML4-ALK fusion gene
  • Detection method and detection kit for EML4-ALK fusion gene
  • Detection method and detection kit for EML4-ALK fusion gene

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Embodiment Construction

[0018] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0019] It should be noted that when a component is said to be "fixed" to another component, it can be directly on the other component or there can also be an intervening component. When a component is said to be "connected" to another component, it may be directly connected to the other component or there may be intervening components at the same time. When a component is said to be "set on" another component, it may be set directly on the other component or t...

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Abstract

The invention discloses a detection method for an EML4-ALK fusion gene. The detection method comprises the following steps: extracting a sample DNA; designing a specific primer used for amplifying a fragment of the EML4-ALK fusion gene; obtaining a target fragment through the single-tube multiplex PCR amplified reaction; designing a single-base extension primer used for the fragment of the EML4-ALK fusion gene; extending a single base of the fragment of the EML4-ALK fusion gene; and conducting mass spectrometry on the nucleic acid to measure a target DNA sequence, so that the EML4-ALK fusion gene of a non-small cell lung cancer patient is effectively detected, and clinical and research foundations are laid for the personalized accurate diagnosis and treatment of the patient. The inventionfurther relates to a detection kit for the EML4-ALK fusion gene.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a detection method and a detection kit of an EML4-ALK fusion gene. Background technique [0002] The fusion of EML4 (Echinodermata microtubule-binding protein-like 4) gene and ALK (Anaplastic Lymphoma Kinase) gene is a common type of fusion in non-small cell lung cancer (NSCLC), and EML4-ALK fusion can lead to the tyrosine kinase of ALK Sustained high expression, thereby activating downstream signaling pathways, leading to tumorigenesis and metastasis. A number of studies have shown that the frequency of EML4-ALK gene fusion in NSCLC is generally 3-11%, and it is mostly related to adenocarcinoma, non-smoking and light smokers, and young patients. [0003] The targeted drug crizotinib has a significant effect on ALK-positive NSCLC patients. Clinical studies have shown that in patients with advanced ALK-positive NSCLC, the objective response rate of crizotinib can reach 50-61%. The Ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158
Inventor 田军龙王文忠胡军张为陈苏平
Owner 为康(苏州)基因科技有限公司
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