Transgenic rape standard sample and establishment method thereof

A standard sample and method establishment technology, which is applied in the preparation of test samples, sampling, and measuring devices, can solve the problems of not being able to meet inspection and quarantine work in a timely manner, long turnaround time, and expensive prices, and achieve slow detection speed, The effect of fast detection speed and low requirement level

Inactive Publication Date: 2018-12-14
HUNAN AGRICULTURAL UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The more serious problem is that there are only a few varieties of standard materials such as genetically modified soybean flour and genetically modified corn flour MON810 in foreign countries. Step up the development of molecular DNA reference standard materials for the detection of genetically modified products, which are used for quantitative detection of genetically modified products; in 2005, we took the lead in developing the national secondary standard materials for genetically modified soybean powder in China, which filled the gap in my country's standard samples for the detection of genetically modified ingredients. Blank, but standard samples such as transgenic rapeseed are still blank

Method used

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Embodiment Construction

[0041] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0042] The invention provides a technical solution:

[0043] The standard sample of transgenic rapeseed and its establishment method are prepared by the following methods:

[0044] (1), take Xiangyou 15 rapeseed and put it in a mortar, add ether and polyethylene powder in a fume hood and stir and grind with a pestle, after the ether evaporates, pass it through a 100-mesh standard sieve, and collect the powder for later use;

[0045] (2), add equal mass of water to the powder ...

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Abstract

The invention discloses a transgenic rape standard sample and a establishing method thereof. The method comprises: respectively using transgenic rape or non-transgenic rape as a raw material; grindingand sieving the raw material, and adding water adding; carrying out stirring and standing, and preparing a precipitate into freeze-dried powder; then mixing the two according to a scientific proportion; repeating the process of ''adding water, carrying out stirring and standing, and preparing the precipitate into freeze-dried powder''; and carrying out split sub-packaging and storing. The transgenic rape standard sample and the method provided by the invention have very important practical significance for comprehensively and deeply researching and solving a preparation technology and a stability guarantee technology of a transgenic ingredient detection standard sample, actively developing the development of a transgenic product detection standard sample in China, and supplementing the blank in this measurement field.

Description

technical field [0001] The invention relates to the technical field of transgenic food detection, in particular to a standard sample of transgenic rapeseed and a method for establishing the same. Background technique [0002] In recent years, breakthroughs have been made in the breeding technology of genetically modified crops, and a number of new varieties have been introduced successively, which have played a huge role in improving crop quality and increasing yield. However, there are many debates about the safety of genetically modified foods. The ecological risks of genetically modified crops, possible environmental problems, the impact of food on human health, product labeling, transportation, international trade, and intellectual property rights have attracted worldwide attention. The safety issues of genetically modified products have developed from differences in academic opinions to intellectual property rights, environmental issues, economic issues and even politi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N21/3586
CPCG01N1/286G01N21/3586G01N2001/2866
Inventor 张振乾王晓丹官春云常涛
Owner HUNAN AGRICULTURAL UNIV
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