Peperomia tetraphylla tissue culture regeneration system and construction method thereof
A technology of tissue culture and construction method, which is applied in the field of watercress green tissue culture regeneration system and its construction, can solve the problems of low rooting rate, poor elongation effect of adventitious buds, low rate of differentiation and proliferation of adventitious buds, etc., to reduce production costs, Improve the survival rate and improve the effect of economic benefits
Inactive Publication Date: 2018-12-18
GUANGDONG UNIV OF PETROCHEMICAL TECH
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Problems solved by technology
[0006] (1) The rate of differentiation and proliferation of adventitious buds is low
[0007] (2) Poor elongation effect of adventitious buds
[0008] (3) Adventitious root induction is difficult and the rooting rate is low
Method used
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[0031] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.
[0032] The invention is applicable to the cultivation method of the watercress green regeneration system, improves the survival rate of the watercress green, reduces the production cost, and improves the economic benefit.
[0033] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.
[0034] like figure 1 As shown, the construction method of the watercress green tissue culture regeneration system provided by the embodiments of the present invention comprises the following steps:
[0035] S101: Add different concentrations of plant gro...
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Belonging to the technical field of plant regeneration by tissue culture technique, the invention discloses a peperomia tetraphylla tissue culture regeneration system and a construction method thereof. The construction method includes: adding plant growth regulators of different concentrations into an MS medium, and observing the influence on adventitious bud induction; adding plant growth regulators of different concentrations into an MS medium, and observing the influence on adventitious bud elongation; and adding plant growth regulators of different concentrations into an MS medium, and observing the influence on root induction. By adding plant growth regulators of different concentrations into the medium, the invention can verify the influence on adventitious bud induction, adventitious bud elongation and bud induction of peperomia tetraphylla, and then works out a method suitable for peperomia tetraphylla regeneration system culture, improves the survival rate of peperomia tetraphylla, lowers the production cost and improves economic benefits.
Description
technical field [0001] The invention belongs to the technical field of plant regeneration through tissue culture technology, and in particular relates to a watercress green tissue culture regeneration system and a construction method thereof. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Watercress green is mostly used for asexual propagation by cuttings and divisions, but these methods have poor seedling uniformity, low multiplication coefficient, and are limited by factors such as seasons and cannot meet the needs of large-scale production. Asexual reproduction is mainly based on cuttings and divisions. Cuttings can be cut by stems. Take cuttings of about 5cm, and keep 1-2 leaves on the upper part. After the cut is dried, insert it into a moist sand bed. Leaf cuttings can also be carried out, take the leaves with petioles, dry them slightly and insert them obliquely on the sand bed (the cutting...
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CPCA01H4/001A01H4/008
Inventor欧阳乐军李莉梅杨培苑陈凯钊孙同川程欣欣胡健文戴发
OwnerGUANGDONG UNIV OF PETROCHEMICAL TECH