Application of radix tetrastigme extracts to preparation of acne removing and youthful look retaining gel
A technology of cloverleaf and extract, applied in the field of daily skin care products, can solve problems such as unsuitability for popularization and use, side effects, etc., and achieve the effects of significant acne-clearing effect, less addition, and promotion of collagen synthesis
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[0036] Preparation of Clover extract: Get 50g of dried root powder of Clover, add 800mL of deionized water, ultrasonically dissolve until fully dissolved, then filter by vacuum filtration, and concentrate the gained filtrate through a rotary evaporator. Carry out vacuum concentration under the conditions of 110° C. and a vacuum degree of 0.096 MPa until the relative density is 3.17-5.31 to obtain the trifolium leaf extract.
[0037] (1) HPLC detection
[0038] Adopt UPLC-MS method to carry out high performance liquid phase detection to the Cloverleaf extract that embodiment 1 obtains, and concrete detection condition and method are:
[0039] (1) Chromatographic conditions: Waters CORTECS C 18 (2.1mm×100mm, 1.6μm); mobile phase: solvent A (acetonitrile) and solvent B (0.1% formic acid water), gradient elution (0 ~ 0.5min, solvent A8% ~ 10%; 0.5 ~ 2.5min, solvent A10%~15%; 2.5~4.5min, solvent A15%~20%; 4.5~6.5min, solvent A20%~40%; 6.5~8.5min, solvent A (40%~60%), flow rate: 0...
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