Method for constructing animal model of epilepsy

A technology for epilepsy animals and mice, applied in the fields of biochemical equipment and methods, chemical instruments and methods, animal/human proteins, etc., to achieve the effect of improving the pathogenesis of epilepsy and having a long life cycle

Active Publication Date: 2018-12-18
THE SECOND HOSPITAL OF HEBEI MEDICAL UNIV
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  • Claims
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  • Method for constructing animal model of epilepsy
  • Method for constructing animal model of epilepsy
  • Method for constructing animal model of epilepsy

Examples

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Embodiment 1

[0031] Example 1 Method for Conditional Knockout of FGF9 Gene in GABAergic Neurons and Construction of Epilepsy Animal Model

[0032] First, FGF9 fl / fl The mouse preparation steps are as follows:

[0033] 1) Construction of a targeting vector for conditional knockout of FGF9

[0034] Analyze the structure of the FGF9 gene, exon1 (ENSMUSE00000337574) can be conditionally knocked out, the FGF9 gene is not expressed after knockout, and the loxP sites at the 5' end and 3' end are inserted in the promoter region and the non-conserved region of intron2, respectively;

[0035] 2) Electroporation of ES cells and screening of ES cell clones with correct recombination

[0036] After the targeting vector was linearized by restriction enzyme digestion, the ES cells were electroporated twice, and a total of 600 clones were selected. After screening by PCR and Southern blot, a total of 10 ES cell clones with correct recombination at both ends were screened;

[0037] 3) Microinjection of ES...

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Abstract

The invention relates to a method for constructing an animal model of epilepsy, belonging to the technical field of construction of animal models. The method comprises the steps of mating FGF9fl/fl mice with GABAergic neurons Cre mice to obtain FGF9fl/+VGAT-Cre hybrid knockout mice, and then mating the obtained hybrid knockout mice with FGF9fl/fl homozygous mice to obtain FGF9 homozygous knockoutmice (FGF9fl/flVGAT-Cre), namely, the animal model of epilepsy. The animal model of epilepsy is closer to the symptoms and mechanisms of clinical epilepsy, and provides a good research tool for the study of the etiology of clinical epilepsy as well as further diagnosis and treatment.

Description

technical field [0001] The invention relates to a method for constructing an animal model, and more particularly relates to a method for constructing an animal model of epilepsy, which belongs to the technical field of animal model construction. Background technique [0002] Epilepsy is a group of chronic diseases and syndromes of sudden, short-lived and recurrent central nervous system (Central nervous system, CNS) dysfunction caused by abnormal excessive discharge of brain neurons. One of the neurological diseases. According to the estimates of the World Health Organization, there are more than 70 million epilepsy patients in the world, accounting for about 1% of the world's population. The prevalence of epilepsy in my country is between 4% and 7%. There are about 6 million active epilepsy patients, and about 400,000 new epilepsy patients each year. Epilepsy can seriously reduce the quality of life of patients, increase the burden on patients' families and society, and t...

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Application Information

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IPC IPC(8): C12N15/85A01K67/027
CPCA01K67/027A01K2217/075A01K2227/105A01K2267/0356C07K14/50C12N15/8509C12N2800/107C12N2800/30
Inventor 李春岩郭默然段伟松伊乐李忠尧刘亚坤毕悦李媛媛张杰
Owner THE SECOND HOSPITAL OF HEBEI MEDICAL UNIV
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