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A method for preparing sulforaphane microcapsules by emulsification spray drying method

A spray-drying method and sulforaphane technology, applied in food drying, application, food forming, etc., can solve the problem of poor improvement of sulforaphane stability, difficult operation process, high toxicity of curing agent formaldehyde, etc. problems, to achieve the effect of solving stability problems, simple equipment, and uniform size

Active Publication Date: 2021-08-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it only disperses sulforaphane in the capsule material and sprays it dry directly. The amount of capsule material used is large, and the effect of improving the stability of sulforaphane is not good.
In CN107569470A, jelly jelly and gelatin with good physical properties are used as capsule materials, and sulforaphane microcapsules are prepared by composite coacervation method. This process is sensitive to temperature and pH, and the operation process is difficult, and the curing agent formaldehyde used is highly toxic

Method used

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  • A method for preparing sulforaphane microcapsules by emulsification spray drying method
  • A method for preparing sulforaphane microcapsules by emulsification spray drying method
  • A method for preparing sulforaphane microcapsules by emulsification spray drying method

Examples

Experimental program
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Effect test

Embodiment 1

[0030] (1) Accurately weigh 2.0 kg of broccoli seeds, put them into a crusher to crush, sieve to a mesh number of 40 mesh, and feed broccoli seed powder to supercritical CO 2 In the extraction kettle, control the extraction pressure to 30MPa, the extraction temperature to 40°C, the extraction time to 4h, CO 2 The flow rate is 7BV / h, and broccoli seed defatted powder is obtained by separation; the defatted powder is added to 10BV water, and endogenous enzymes are used to hydrolyze to obtain the sulforaphane extract; The volume ratio between the element extract and DM850 resin is 1:22ml, the ratio of diameter to height of the resin column is 1:8, and the flow rate is 4BV / h to pass through the resin column. % ethanol to remove some impurities, then elute with 6BV of 50% ethanol, collect the eluate and concentrate in vacuo; after concentrating to no alcohol smell, add 2BV of ethyl acetate to extract twice, and concentrate in vacuo to remove the organic solvent.

[0031] (2) Take ...

Embodiment 2

[0035] (1) Accurately weigh 2.0 kg of broccoli seeds, put them into a crusher and crush them, sieve to a mesh number of 60 mesh, and feed broccoli seed powder to supercritical CO 2 In the extraction kettle, control the extraction pressure to 25MPa, the extraction temperature to 40°C, the extraction time to 2h, CO 2 The flow rate is 8BV / h, and broccoli seed defatted powder is obtained by separation; the defatted powder is added to 10BV water, and endogenous enzymes are used to hydrolyze to obtain the sulforaphane extract; The ratio of prime extract to SP850 resin is 1:22ml by volume, the diameter-to-height ratio of the resin column is 1:8, and the flow rate is 4BV / h to pass through the resin column. % ethanol to remove some impurities, then elute with 6BV of 50% ethanol, collect the eluate and concentrate in vacuo; after concentrating until there is no alcohol smell, add 2BV of dichloromethane to extract twice, and concentrate in vacuo to remove the organic solvent.

[0036](2...

Embodiment 3

[0040] (1) Accurately weigh 2.0 kg of broccoli seeds, put them into a crusher and crush them, sieve to a mesh number of 20 mesh, and feed broccoli seed powder to supercritical CO 2 In the extraction kettle, control the extraction pressure to 40MPa, the extraction temperature to 30°C, the extraction time to 4h, CO 2 The flow rate is 6BV / h, and broccoli seed defatted powder is obtained by separation; the defatted powder is added to 10BV water, and endogenous enzymes are used to hydrolyze to obtain the sulforaphane extract; The volume ratio between the element extract and DM850 resin is 1:22ml, the ratio of diameter to height of the resin column is 1:8, and the flow rate is 4BV / h to pass through the resin column. % ethanol to remove some impurities, then elute with 6BV of 50% ethanol, collect the eluate and concentrate in vacuo; after concentrating until there is no alcohol smell, add 2BV of n-butanol to extract twice, and concentrate in vacuo to remove the organic solvent.

[0...

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Abstract

The invention discloses a method for preparing sulforaphane microcapsules by an emulsification spray-drying method. Broccoli seeds are used as raw materials, degreased by supercritical methods after crushing, hydrolyzed with endogenous enzymes to extract sulforaphane, and macroporous resin and organic Purify sulforaphane by solvent extraction, then add emulsifier, antioxidant and oil to sulforaphane to prepare oil phase, add polyethylene glycol and water to composite capsule material to prepare water phase, and stir in water phase according to the core material ratio While adding the oil phase, high-speed shearing, and finally spray-drying the obtained emulsion to obtain sulforaphane microcapsules. The sulforaphane microcapsules prepared by the invention have uniform size and smooth surface; the embedding rate reaches 80.59%, and the particle size of the microcapsules is 1.03-3.38 μm; the retention rate of sulforaphane can reach more than 90 percent. The method of the invention has simple process, low production cost, improves the tolerance of sulforaphane to high temperature, and is suitable for industrial application.

Description

technical field [0001] The invention belongs to the field of food, and relates to a method for preparing sulforaphane microcapsules by using an emulsification spray drying method. Background technique [0002] Sulforaphane [1-isothiocyanate (4R)-4-(methylsulfinyl) butane, Sulforaphane] is the hydrolysis product of glucoraphanin, a yellow oily liquid, easily soluble in methanol, acetonitrile, acetic acid In organic solvents such as ethyl ester. Sulforaphane, commonly found in cruciferous plants, can prevent, delay or reverse precancerous lesions by inducing type II detoxification enzymes. Studies have found that sulforaphane can not only inhibit and kill cancer cells, but also has anti-oxidation, immune regulation, anti-inflammation, and prevention of cardiovascular diseases. This makes sulforaphane one of the most concerned natural products in the world. However, sulforaphane is unstable and is easily affected by environmental factors such as temperature, oxygen, light, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/105A23P10/30
CPCA23V2002/00A23L33/105A23P10/30A23V2250/21A23V2300/10A23V2300/14
Inventor 栾连军黄忆真刘雪松吴永江谈满良
Owner ZHEJIANG UNIV