Use of Niacinamide in Prevention of Salivary Gland Radiation Injury
A technology for radiation damage and salivary glands, which is applied in the field of medicine and health, can solve the problems of short-term effect and difficulty in application, and achieve the effects of preventing radiation damage, improving survival rate, and reducing cell apoptosis
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Embodiment 1
[0027] Example 1: Experimental research on the prevention of radiation injury of submandibular gland cells of neonatal rats by NAM
[0028] 1. Purpose
[0029] To explore the prevention and treatment effect of NAM on radiation injury of submandibular gland cells in rats.
[0030] 2. Materials and methods
[0031] In this example, primary cultured neonatal rat submandibular gland cells in the third-generation logarithmic growth phase were randomly divided into blank group (Control, abbreviated as Cont), pure NAM group (NAM), simple radiation group (IR) and NAM pretreatment group (NAM+IR), 5GyX-ray irradiation with RS2000 biological X-ray irradiator (before irradiation, NAM intervention group (pure NAM group and NAM pretreatment group) were pretreated with 50μM and 200μM NAM for 30min respectively, of which 50μM NAM pretreatment for 30 min: pre-incubating primary cultured submandibular gland cells of neonatal rats with 50 μM nicotinamide for 30 min; Radiation group) only give...
Embodiment 2
[0039] Example 2: Experimental research on the prevention of radiation injury of rat submandibular gland tissue by NAM
[0040] 1. Purpose
[0041] To explore the prevention and treatment effect of NAM on radiation injury of submandibular gland tissue.
[0042] 2. Materials and methods
[0043]In this study, 24 Wistar rats (body weight 180-230g, male or female) were randomly divided into blank group (Control, abbreviated as Cont), pure NAM group (NAM), simple radiation group (IR) and NAM pretreatment group ( NAM+IR), with RS2000 biological X-ray irradiator line 20GyX-ray irradiation (half an hour before irradiation, NAM intervention group (simple NAM group and NAM pretreatment group) were given NAM500mg / kg by intragastric administration, control group (blank group) group and simple radiation group) were only given an equal volume of normal saline). Seven days later, the submandibular gland tissue was taken under anesthesia, paraffin sections were made, and HE staining was p...
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