Method for acquiring giant panda fed food formula based on intestinal microflora detection and feeding method
A technology of intestinal microbes and feeding methods, applied in the field of giant panda breeding, can solve problems such as indigestion prone to occur, and achieve the effect of solving indigestion and improving the distribution of intestinal microbes
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[0032] A method for obtaining food formula for giant pandas based on intestinal microbial detection, including sample collection, microbial extraction, microbial identification and functional analysis, and providing feeding methods, specifically including:
[0033] (1) Sampling: At the Chengdu Research Base of Giant Panda Breeding, healthy and non-pregnant giant pandas were divided into three groups: eating bamboo shoots, bamboo stems, and bamboo leaves. For fresh feces samples from pandas in four seasons, peel off a layer of feces that is in contact with the ground, put them into sterile bags, vent the air, transport them back to the laboratory in an ice box, and store them at -80°C;
[0034] (2) Microbiological identification and functional analysis of collected feces: using German The company’s DNA kit extracts the collected feces, uses Illumina HiSeq 2500 for sequencing, and compares them with wild giant pandas, and finally obtains the comprehensive intestinal microbial c...
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