Method for acquiring giant panda fed food formula based on intestinal microflora detection and feeding method
A technology of intestinal microbes and feeding methods, applied in the field of giant panda breeding, can solve problems such as indigestion prone to occur, and achieve the effect of solving indigestion and improving the distribution of intestinal microbes
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[0032] A method for obtaining a feeding formula for giant pandas based on intestinal microbial detection, including sample collection, microbial extraction, microbial identification and functional analysis, and provision of feeding methods, including:
[0033] (1) Sampling: conducted at the Chengdu Research Base of Giant Panda Breeding. The healthy and unconceived giant pandas were divided into 3 groups of feeding bamboo shoots, bamboo stems, and bamboo leaves. After 20 days of continuous feeding, the 3 groups were collected. Fresh panda feces samples from the four seasons, peel off a layer of feces in contact with the ground, put them in a sterile bag, vent the air, transport them back to the laboratory in an ice box, and store them at -80°C;
[0034] (2) Microbiological identification and functional analysis of collected feces: use Germany The company’s DNA kit extracts the collected feces, uses Illumina HiSeq 2500 for sequencing, and compares them with giant pa...
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