A method for quickly focusing and photographing plane samples

Abstract

The invention discloses a method for quickly focusing and photographing a plane sample, belonging to the technical field of sample imaging. It divides the plane to be focused and photographed of a plane sample into a plurality of unit areas, preferably three discrete points, and calculates each discrete point respectively. The focus distance of the point, and then calculate the focus distance of each unit area by interpolation method, and then combine the focus distance of each unit area to control the rapid movement of the lens, effectively realizing the rapid focus of the lens and the sample area, so that there is no need to adjust the lens every time The focal length can be used to complete accurate photography of each unit area. The method for quickly focusing and photographing plane samples of the present invention has simple steps and easy operation, effectively reduces the focusing time between the lens and the sample area, improves the efficiency of focusing and photographing plane samples, and ensures the clarity of focusing and photographing plane samples. The cost of focusing and taking pictures of samples is reduced, and the method has good promotion and application value.

Description

technical field [0001] The invention belongs to the technical field of sample imaging, and in particular relates to a method for quickly focusing and photographing a plane sample. Background technique [0002] In the field of biomedicine, microscopic scanning and imaging of various samples is often required, such as single-celled organisms, small algae, bacterial culture fluid, loose tissues of animals and plants, testis, anthers, blood, pus, skin tissue fluid, pharyngeal wall, etc. Various samples are often fixed on medical slides first, and then the slides are placed and fixed on the stage of the microscopic scanning imaging device. The movement in the direction makes the sample pass through the focal plane of the lens in turn, and each point on the sample is imaged through the lens, and then the images of each point are stitched together to form an image of the entire medical sample. [0003] At present, when using a microscopic scanning imaging device to scan a sample, ...

AI Technical Summary

Problems solved by technology

However, when using the existing micro-scanning imaging method to achieve high-definition imaging of the sample, the lens of the device is often required to automatically and accurately focus on each area divided by the sample plane, and then complete the corresponding photography of each area. At present, it usually takes at least 3 seconds for the lens to automatically and accurately focus on each area, and there are usually no less than 800 m×n areas, that is, the time for focusing on the sample plane will be no less than 40 minutes, which makes the sample scan The time is too long, even if you work 8 hours a day, you can only scan and image about 12 samples, which is lower than the efficiency of manual operation (about 25 samples / day), which greatly restricts the efficiency of sample scanning and imaging, and improves the efficiency of sample scanning. The scanning cost of high-definition scanning restricts the rapid scanning and imaging of samples, which cannot fully meet the needs of fast and accurate imaging of samples, and has great application limitations.

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