Application of Diketopyrrolopyrrole Compounds/Tetraphenylethylene Compounds Composite Nanoparticles in the Detection of Hypochlorite Ions

A technology of diketopyrrolopyrrole and composite nanoparticles, which is applied in nanotechnology, nanotechnology, nanotechnology for materials and surface science, etc., to achieve strong fluorescence emission, which is conducive to large-scale production applications, high selectivity and Effect of Sensitivity Ratiometric Assays

Active Publication Date: 2019-12-10
CENT SOUTH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, inorganic heavy metal materials are mostly used in the preparation of FRET nanoprobes, and their potential safety threats are an unavoidable problem.

Method used

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  • Application of Diketopyrrolopyrrole Compounds/Tetraphenylethylene Compounds Composite Nanoparticles in the Detection of Hypochlorite Ions
  • Application of Diketopyrrolopyrrole Compounds/Tetraphenylethylene Compounds Composite Nanoparticles in the Detection of Hypochlorite Ions
  • Application of Diketopyrrolopyrrole Compounds/Tetraphenylethylene Compounds Composite Nanoparticles in the Detection of Hypochlorite Ions

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Preparation of FRET nanoparticles based on thienylpyrrolopyrrole diketopic derivatives. Such as figure 1 As shown, this embodiment uses two kinds of alkoxy (oligopolyethylene glycol) modified fluorescent molecules (NDPP and TPE1, both molecular formulas are as follows figure 1 shown) as raw materials to prepare FRET nanoparticles. In water, add two fluorescent compounds at a ratio of 1:4 of NDPP:TPE1 to make the total concentration 50 μM / L. After ultrasonication for 1 minute, the desired composite nanoparticles were prepared. Such as figure 2 As shown, the left figure shows the particle size and morphology of FRET nanoparticles measured by transmission electron microscopy (TEM), indicating that co-assembly of NDPP and TPE1 can obtain spherical regular particles. figure 2 The middle right panel shows the fluorescence emission map of the nanoparticles. By comparison, it can be seen that after mixing with NDPP, the fluorescence intensity of TPE1 particles is about 8...

Embodiment 2

[0038] FRET nanoparticles on ClO - The fluorescence emission spectrum of the response is as image 3 shown. In aqueous solution, FRET nanoparticles FRET nanoparticles have emission peaks at 490nm and 554nm, which correspond to the characteristic fluorescence emission of TPE1 and NDPP, respectively. With ClO - With the addition of , the fluorescence of TPE1 gradually increased, while that of NDPP gradually decreased. pass image 3 The slope of the fitted curve obtained in the right figure in the middle can be calculated, the FRET nanoparticles in water to ClO- The detection limit can reach 92nM, which can be used for ClO in water environment - real-time monitoring.

Embodiment 3

[0040] FRET nanoparticles on ClO - The specificity of the response is examined as Figure 4 shown. Prepare 8 parts of aqueous solution of FRET nanoparticles with PBS buffer, number 1-8. Then add water, H 2 o 2 , · Oh, 1 o 2 ,NO · ,ONOO - ,t-BuOOH and ClO - . The spectrum shows that: in addition to ClO - In addition, other reactive oxygen species had almost no effect on the fluorescence emission of the solution. This comparative experiment shows that the FRET nanoparticles in the present invention are in ClO - Good selectivity in detection.

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Abstract

The invention discloses an application of diketopyrrolopyrrole compound / tetraphenylethylene compound composite nanoparticles in detection of hypochlorite ions. The composite nanoparticles have FRET (fluorescence resonance energy transfer) effect and good water solubility, can realize high-sensitivity and high-selectivity ratio detection of ClO<-> in a water system or fluorescence imaging of ClO<->in cells, and can be widely applied to the fields of biological detection, imaging and the like.

Description

technical field [0001] The invention relates to a nanoparticle with fluorescence energy transfer, in particular to a composite nanoparticle with fluorescence resonance energy transfer effect (FRET) assembled from a diketopyrrolopyrrole compound and a tetraphenylethylene compound, which is used for ClO in water - Detection or imaging of the intracellular environment to achieve ClO - An efficient detection method belongs to the field of biological detection. Background technique [0002] Small molecules of biologically active oxides play an important role in physiology and pathology, and their concentration levels reflect the physiological and pathological state of cells. Among them, hypochlorite, as an important active oxide in organisms, participates in many physiological processes in organisms and is beneficial to health It plays an important role, but excessive hypochlorous acid can cause various tissue damage and diseases including cardiovascular disease and lung injury...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D487/04C09K11/06G01N21/64B82Y30/00B82Y40/00
CPCB82Y30/00B82Y40/00C09K11/06C09K2211/1007C09K2211/1029C09K2211/1092C09K2211/1425G01N21/643
Inventor 刘正春聂凯旋梁波石环环董波朝乐蒙龙孟秋徐慧
Owner CENT SOUTH UNIV
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