Chicken Nrf2 protein antibody and immunogen thereof, immunogenic polypeptide, detection kit and application
A technology of immunogenic polypeptide and detection kit, applied in the field of virus immunology
Inactive Publication Date: 2019-05-21
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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Abstract
The invention discloses a chicken Nrf2 protein antibody and immunogen thereof, immunogenic polypeptide, a detection kit and application, and belongs to the technical field of viral immunology. Aimingat the situation that in the prior art, an antibody for effectively detecting the expression condition of chicken Nrf2 protein and a related detection kit are in lack, the immunogenic polypeptide is provided, the amino acid sequence of the immunogenic polypeptide is shown as SEQ ID NO:1. The immunogenic polypeptide is coupled with carrier protein through a coupling agent, the immunogen is obtained, the immunogen and an adjuvant are mixed to immunize an inoculated rabbit, the blood of the immunize rabbit is taken to be purified, the chicken Nrf2 protein antibody is prepared, and the ELISA detection kit for detecting the chicken Nrf2 protein is provided. The detection kit can be used for monitoring the expression and distribution of the chicken Nrf2 protein in cells.
Application Domain
Serum immunoglobulinsImmunoglobulins against animals/humans +3
Technology Topic
Viral immunologyImmunogenicity +7
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