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Application of rice osracd gene in improving rice panicle length

A rice, gene technology, applied in the fields of agriculture and molecular biology, which can solve the problem that the connection has not been reported yet

Active Publication Date: 2022-05-27
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

OSR ( OsRac5 ) was isolated from young rice panicles [11] , existing studies have shown that OSR Gene may be involved in regulation of rice fertility [12] , but the relationship between this gene and panicle length has not been reported

Method used

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  • Application of rice osracd gene in improving rice panicle length
  • Application of rice osracd gene in improving rice panicle length

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Experimental program
Comparison scheme
Effect test

Embodiment

[0029] Step S1: Rice OsRacD Construction of gene RNA interference vector: TRIzol method was used to extract total RNA of rice young panicle, and reverse transcription was used to prepare rice young panicle cDNA. Primer P1: 5'-CACCAGCCTCCAAAGCAAAAG-3' and primer P2: 5'-AGAGACCAACACCACAAAGG-3', primer P1 It contains the protective base CACC, and was amplified by PCR using the cDNA of rice young panicle as a template. OsRacD Gene interference fragment, the PCR product was connected to the corresponding site of the entry vector pENTR / D gateway (purchased from Invitrogen), the ligation product was transformed into TOP10 competent cells, and the transformants were screened on LB plates containing kanamycin and confirmed by sequencing named after pENTR- OsRacD , pENTR- OsRacD The vector was subjected to LR reaction with the pANDA vector, and then the ligation product was transformed into E. coli DH5α competent cells, and positive clones were screened on LB plates containing kanamyc...

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Abstract

The invention discloses rice OSR The application of the gene in increasing the ear length of rice belongs to the technical field of agriculture and molecular biology. The main points of the technical scheme of the present invention are: rice OSR The application of genes in improving rice panicle length, the rice OSR The nucleotide sequence of the gene is shown in SEQ ID NO: 1, first construct rice OSR The RNA interference plant expression vector of the gene is transformed into rice callus by Agrobacterium-mediated method, induced to differentiate into regenerated rice plants, and then screened generation by generation until the T2 generation is obtained OSR Genetic RNA interference in transgenic rice. The present invention constructs OSR Gene RNA interference plant vector, using Agrobacterium-mediated method to transform rice callus to obtain T2 generation OSR Gene RNA interference transgenic rice, agronomic statistics show that compared with the control, the panicle length of the transgenic rice increased by 7.22%-7.51%, and the difference reached a significant level.

Description

technical field [0001] The invention belongs to the technical field of agriculture and molecular biology, in particular to rice OsRacD Application of genes in improving ear length in rice. Background technique [0002] Rice ( Oryza sativa L.) is an important food crop, feeding more than half of the global population. my country is a big country of rice production and consumption, so rice plays a vital role in the production and safety of food in our country and even in the world [1] . The growth and development and physiological regulation of rice also affect the final yield and quality. Therefore, the use of genetic engineering technology to carry out scientific and technological breeding of rice in order to enhance the quality of rice and improve the yield has become an effective way to improve the level of rice production. [2] . [0003] The composition of rice yield is closely related to various panicle traits of rice [3] , ear length is an important component of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H5/02A01H6/46
CPCC12N15/8218C12N15/8261C07K14/415
Inventor 梁卫红王美娜彭静静王凯婕安文静黄俊骏刘肖飞刘亚菲
Owner HENAN NORMAL UNIV