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Construction method of lactococcus lactis MG1363 and application of lactococcus lactis MG1363 in treating bacterial vaginitis

A technology of MG1363, Lactococcus lactis, applied in bacteria, antibacterial drugs, medical preparations containing active ingredients, etc., can solve problems such as inability to meet tissue repair

Inactive Publication Date: 2019-01-01
NANCHANG UNIV
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Problems solved by technology

However, CXCL12 is easily degraded by dipeptide aminopeptidase IV (DPP IV) in human blood, and the endogenous CXCL12 level produced during the repair of severe tissue damage cannot meet the needs of tissue repair, so it is necessary to introduce exogenous CXCL12

Method used

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  • Construction method of lactococcus lactis MG1363 and application of lactococcus lactis MG1363 in treating bacterial vaginitis
  • Construction method of lactococcus lactis MG1363 and application of lactococcus lactis MG1363 in treating bacterial vaginitis
  • Construction method of lactococcus lactis MG1363 and application of lactococcus lactis MG1363 in treating bacterial vaginitis

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Experimental program
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Effect test

Embodiment 1

[0055] Detailed description of the embodiment: the drug effect is evaluated by a rat model of bacterial vaginosis

[0056] 1. Establishment of rat vaginitis model

[0057] (1) The experimental groups were negative control group, model group, metronidazole treatment, Lactococcus lactis engineering strain treatment group, and Lactobacillus crispatus engineering strain treatment group, with 5 animals in each group;

[0058] (2) Antibiotic treatment, cut the medical absorbent gelatin sponge into a size of 0.5cm×0.5cm, absorb 50 μL of antibiotics with a concentration of 100mg / mL; after the gelatin is fully absorbed and saturated with antibiotics, insert the gelatin sponge containing antibiotics into the vaginal plug and intrauterine cavity; antibiotics were treated for 6 times, and the treatment was continued for three days and recovered once;

[0059] (3) After antibiotic treatment, the B-hemolytic streptococcus, Staphylococcus aureus, and Escherichia coli were prepared into a mi...

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Abstract

The invention discloses construction method of lactococcus lactis MG1363 and application of the lactococcus lactis MG1363 in treating bacterial vaginitis. The construction method comprises the following steps of firstly, chemically synthesizing human and mouse chemokine CXCL12, also called stromal cell derived factor-1 (SDF-1) and belonging to small molecular proteins of a CXC chemokine family; and then adding specific secretory expression cell-penetrating peptide SPusp45 of the lactococcus lactis, constructing pMG36e-CXCL12 recombinant plasmids, and then enabling the pMG36e-CXCL12 recombinantplasmids to enter the lactococcus lactis MG1363 with electricity facing to realize secretory expression of chemokine CXCL12. The chemokine CXCL12 is continuously expressed with a manner of vagina embolization administration, so that the effects of remission and treatment to the bacterial vaginitis are realized.

Description

technical field [0001] The invention relates to a preparation method of Lactococcus lactis MG1363 and its application in bacterial vaginosis, belonging to the field of biopharmaceuticals. Background technique [0002] Chemokines are a class of small-molecule polypeptide substances that have directional chemotaxis and recruitment effects on leukocytes and stem cells. Their structures and functions are similar, and most of them have four conserved cysteines in the amino acid sequence. Depending on the number and spacing of N-terminal cysteines, they are divided into C-, CXC-, CC- and CX3C-4 chemokines. Chemokine CXCL12, also known as stromal cell-derived factor-1 (SDF-1), has two forms, SDF-1α / CXCL12a and SDF-1β / CXCL12b. CXCR4 is a specific receptor for the chemokine CXCL12, which belongs to the G protein-coupled transmembrane receptor family and has a high affinity with CXCL12. It is widely expressed in blood, immune and central nervous system cells. Studies have shown that...

Claims

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Application Information

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IPC IPC(8): C12N15/74C12N1/21A61K35/744A61P31/04A61P15/02C12R1/01
CPCA61K35/744A61P15/02A61P31/04C07K14/521C12N15/746
Inventor 陈廷涛田溥塬
Owner NANCHANG UNIV
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