A closed internal circulation trace plant tissue water efficient extraction device
A plant tissue and extraction device technology, applied in sampling devices and other directions, can solve the problems of toxic experimental results of azeotropes, difficulty in effectively collecting water, and low extraction efficiency, and achieve the effects of simple cost, high speed, and high extraction efficiency.
Active Publication Date: 2021-12-14
GANSU AGRI UNIV
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Problems solved by technology
[0008] The technical problem to be solved by the present invention is: to solve the problems faced by the current hydrogen and oxygen isotope labeling technology in the soil-plant-atmosphere continuum (SPAC), the equipment is expensive, the extraction efficiency is low, it is difficult to effectively collect the extracted water, the azeotrope is toxic and Because it often contains hydrogen and oxygen elements, it will affect the experimental results and other key issues
Method used
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Experimental program
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Embodiment 1
[0034] Add the ice-water mixture to the 1 / 3 place of the settling tube 14 in the 1L beaker 13, add 0.8 g of chopped plant tissue in the test tube 9 with an outer diameter of 18 mm, add water to the 3 / 4 place of the test tube in the 1L beaker 8 The water in the beaker 8 is boiled by heating with the electric furnace 6, and at the same time, the power switch 19 is turned on to make the air pump 21 run, so as to promote the directional circulation of the gas inside the device. After 1 hour, turn off the electric furnace 6 and the power supply 18 of the 0.28L / min medical micro-pump 21, take out the settling tube 14 with an inner diameter of 18mm and shake it with your wrist to make the water condensed on the wall of the settling tube 14 accumulate to the bottom, and finally Collect the extracted water in the settling tube 14 with a 1 mL pipette or syringe.
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Abstract
The invention discloses a device for efficiently extracting moisture in a closed internal circulation trace plant tissue, which includes a heating evaporation device, a condensation device and a power device; rubber stopper, the first glass conduit, the second glass conduit and the first support, the test tube is fixed on the first support, the test tube is placed inside the first beaker, the second beaker is placed on the second support, the The settling tube is fixed on the third support, and the settling tube is placed in the second beaker; the power device is composed of a power supply, a switch, a wire, and a medical micro-pump; the plant tissue consumption is small, and the extraction efficiency is high and the speed is fast. Multiple extraction tests found that this method can extract more than 98% of the water in 0.8g of shredded apple leaves, roots and branches in 1 hour, and the water extraction rate of 0.3g of shredded apple leaves can reach 99.41%. Test requirements for isotope abundance.
Description
Technical field: [0001] The invention relates to a closed-type internal circulation trace plant tissue water efficient extraction device. Background technique: [0002] Hydrogen and oxygen stable isotopes have been widely used in many fields of ecology, providing a very effective method for SPAC water utilization sources. In the SPAC hydrogen and oxygen isotope tracer research, the extraction of soil and plant water is one of the most critical links. The extraction rate of different methods and whether distillation occurs during the extraction process will affect the determination and analysis of hydrogen and oxygen isotopes, directly affecting correctness of the results. Commonly used plant tissue water extraction methods mainly include vacuum distillation, azeotropic distillation and extrusion extraction. [0003] The above three methods have the following disadvantages respectively: [0004] Vacuum distillation requires complex equipment such as vacuum pumps, and has t...
Claims
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IPC IPC(8): G01N1/14
CPCG01N1/14
Inventor 陈佰鸿王宝林毛娟李文芳马宗桓梁国平王颖
Owner GANSU AGRI UNIV