A growth-promoting medium and its application in cultivation of Cordyceps sinensis

A Cordyceps sinensis and growth-promoting technology, applied to a growth-promoting medium and its application field in the cultivation of Cordyceps sinensis, can solve the problem of low yield and the like, and achieve the effects of improving quality and improving medicinal value

Active Publication Date: 2021-11-19
SHAANXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Nowadays, artificial Cordyceps sinensis can be successfully cultivated, but the yield is not high, and an excellent medium for promoting the growth and metabolism of Cordyceps fungi is urgently needed

Method used

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  • A growth-promoting medium and its application in cultivation of Cordyceps sinensis
  • A growth-promoting medium and its application in cultivation of Cordyceps sinensis

Examples

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preparation example Construction

[0040] The preparation method of the astragalus extract is as follows: wash the astragalus root and dry it naturally, crush it to 100 meshes to obtain the astragalus powder, then add 60wt% acetone aqueous solution to the astragalus powder, and the mass volume ratio of the astragalus powder to the 60wt% acetone aqueous solution is 1:8 (g / mL), vibrating leaching at 50°C and 200r / min for 2h, filtered through a 200-mesh filter cloth to obtain filtrate A and astragalus filter residue; add 60wt% ethanol aqueous solution to the obtained astragalus filter residue, The mass volume ratio of the Radix Astragali filter residue and 60wt% ethanol aqueous solution is 1:8 (g / mL), shake and extract at 50°C and 200r / min for 2h, and filter through a 200-mesh filter cloth to obtain filtrate B, Combine filtrate A and filtrate B, and freeze and centrifuge the resulting mixed extract at minus 2°C at a speed of 15,000r / m for 28min. The obtained supernatant is concentrated in a rotary evaporator at 55°...

Embodiment 1

[0046] A growth-promoting medium, comprising the following components by weight: 200 parts of potatoes, 1500 parts of water, 20 parts of agar, 3 parts of protein powder, 20 parts of glucose, 3 parts of potassium dihydrogen phosphate, 1 part of magnesium sulfate, 0.009 parts phytohormone.

[0047] The plant hormone is a mixture of indole acetic acid, 6-benzyl adenine and abscisic acid in a mass ratio of 1:1:1.

[0048] A preparation method for growth-promoting medium, comprising the following steps:

[0049] Ⅰ. Weigh each raw material component by mass parts;

[0050] Ⅱ. Peel the potatoes, remove the bud eyes, cut into 2mm pieces 3 Then add water, heat at 95°C for 30min, filter with 6 layers of 100-mesh gauze, add agar to the filtrate, heat at 95°C for 3min, then add protein powder, glucose, potassium dihydrogen phosphate, magnesium sulfate, Phytohormones, stir evenly, and adjust the pH value to 5.5.

Embodiment 2

[0052] It is basically the same as Example 1, except that the plant hormone is mixed with indole acetic acid and 6-benzyl adenine at a mass ratio of 1:1.

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Abstract

The invention discloses a growth promoting medium. The growth-promoting medium of the present invention mainly includes potatoes, water, agar, protein powder, glucose, potassium dihydrogen phosphate, and magnesium sulfate. Plant hormones or traditional Chinese medicine extracts may also be included. The invention also discloses the application of the growth-promoting medium in the cultivation of Cordyceps sinensis. Compared with the prior art, the growth-promoting medium of the present invention is added with phytohormones and Chinese medicine extracts, which is conducive to inducing the growth of spores and hyphae of Cordyceps sinensis, thereby shortening the period of artificially cultivating Cordyceps sinensis, which has important economic significance; Set a specific temperature control value and light time to increase the content of active substances such as cordycepin. At the same time, because the distance from the origin of the original grass is the closest, the cultivation environment and the growth environment of the original grass can best ensure the maximum active ingredient of the Cordyceps. Separation and cultivation The various functions of the obtained Cordyceps sinensis mycelium are closer to wild Cordyceps sinensis.

Description

technical field [0001] The invention relates to the preparation and improvement of the medium, in particular to a growth-promoting medium and its application in the cultivation of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis (Ophiocordyceps sinensis) is a traditional precious Chinese medicinal material in my country, which is a combination of insects and fungi. The worm is the larva of the bat moth of Cordyceps sinensis, and the bacterium is the fungus of Cordyceps sinensis. The formation of Cordyceps sinensis is that the fungus of Cordyceps sinensis infects the bat moth larvae in summer, and the mycelium absorbs the nutrients in the larvae and gradually fills the worm body until it grows from the head of the worm body in the spring and summer of the second year to form fruiting bodies. Due to the increase in manual excavation activities in recent years, the number of wild Cordyceps sinensis has dropped sharply, and the vegetation in the production a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 卫培峰卫文鑫高峰钟兴林
Owner SHAANXI UNIV OF CHINESE MEDICINE
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