Sustained-release microsphere containing exosomes of mesenchymal stem cells and preparation method thereof

A technology of mesenchymal stem cells and slow-release microspheres, applied in the field of biological stem cells, can solve problems such as long treatment time, difficult endometrial repair, and women's distress, so as to prolong the effect, repair endometrial damage, and survive time long effect

Inactive Publication Date: 2019-01-11
天津欣普赛尔生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The uterus is very important to a woman's life, but once the endometrium is damaged, it will cause a series of consequences. The existing endometrium is difficult to repair and the treatment time is long, which has brought great distress to many women

Method used

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  • Sustained-release microsphere containing exosomes of mesenchymal stem cells and preparation method thereof
  • Sustained-release microsphere containing exosomes of mesenchymal stem cells and preparation method thereof
  • Sustained-release microsphere containing exosomes of mesenchymal stem cells and preparation method thereof

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Embodiment 1

[0021] The invention provides a slow-release microsphere containing mesenchymal stem cell exosomes, which comprises 1 part of mesenchymal stem cell exosome solution, 98 parts of polylactic acid-glycolic acid copolymer solution and 1 part by volume. Parts of polyvinyl alcohol solution, by mass fraction ratio, mesenchymal stem cell exosome solution includes 10-25 parts of exosome freeze-dried powder and 1-4 parts of distilled water, by mass fraction ratio, polylactic acid-glycolic acid copolymer The solution includes 10-25 parts of polylactic acid-glycolic acid copolymer and 75-90 parts of dichloromethane solution. The exosome freeze-dried powder is prepared by starvation culture of endometrial mesenchymal stem cells. The starvation culture time is 12- For 24 hours, autologous endometrial mesenchymal stem cells are extracted from human endometrial mesenchymal stem cells and cultured according to normal culture methods, and the culture time is 48-72 hours.

Embodiment 2

[0023] The invention provides a slow-release microsphere containing mesenchymal stem cell exosomes, which comprises 4 parts of mesenchymal stem cell exosome solution, 92 parts of polylactic acid-glycolic acid copolymer solution and 4 parts by volume. Parts of polyvinyl alcohol solution, by mass fraction ratio, mesenchymal stem cell exosome solution includes 10-25 parts of exosome freeze-dried powder and 1-4 parts of distilled water, by mass fraction ratio, polylactic acid-glycolic acid copolymer The solution includes 10-25 parts of polylactic acid-glycolic acid copolymer and 75-90 parts of dichloromethane solution. The exosome freeze-dried powder is prepared by starvation culture of endometrial mesenchymal stem cells. The starvation culture time is 12- For 24 hours, autologous endometrial mesenchymal stem cells are extracted from human endometrial mesenchymal stem cells and cultured according to normal culture methods, and the culture time is 48-72 hours.

Embodiment 3

[0025] The invention provides a slow-release microsphere containing mesenchymal stem cell exosomes, which comprises 2 parts of mesenchymal stem cell exosome solution, 96 parts of polylactic acid-glycolic acid copolymer solution and 2 parts by volume. Parts of polyvinyl alcohol solution, by mass fraction ratio, mesenchymal stem cell exosome solution includes 10-25 parts of exosome freeze-dried powder and 1-4 parts of distilled water, by mass fraction ratio, polylactic acid-glycolic acid copolymer The solution includes 10-25 parts of polylactic acid-glycolic acid copolymer and 75-90 parts of dichloromethane solution. The exosome freeze-dried powder is prepared by starvation culture of endometrial mesenchymal stem cells. The starvation culture time is 12- For 24 hours, autologous endometrial mesenchymal stem cells are extracted from human endometrial mesenchymal stem cells and cultured according to normal culture methods, and the culture time is 48-72 hours.

[0026] According to...

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Abstract

The invention provides a sustained-release microsphere containing exosomes of mesenchymal stem cells and a preparation method thereof, relating to the technical field of biological stem cells. The sustained-release microsphere containing exosomes of mesenchymal stem cells comprises a mesenchymal stem cell exosome solution, a polylactic acid-Glycolic acid copolymer solution and a polyvinyl alcoholsolution, wherein the mesenchymal stem cell exosomal solution includes exosomal lyophilized powder; the polylactic acid-Glycolic acid copolymer solution comprises polylactic acid-Glycolic acid copolymer and dichloromethane solution. The invention enables the activity and function of exosome to be preserved and utilized, and the survival time can be longer, the effect of exosome can be prolonged, and the endometrium damage can be effectively repaired.

Description

technical field [0001] The invention relates to the technical field of biological stem cells, in particular to a slow-release microsphere containing mesenchymal stem cell exosomes and a preparation method. Background technique [0002] The uterus is very important to a woman's life, but once the endometrium is damaged, it will cause a series of consequences. The existing endometrium is difficult to repair and the treatment time is long, which has brought great troubles to many women. [0003] Mesenchymal stem cells (Mesenchymal Stem Cells, MSC) are pluripotent stem cells, which have multiple functions such as multidirectional differentiation, hematopoietic support, promotion of stem cell implantation, and immune regulation. They mainly exist in the endometrium, fat, and bone marrow. Middle; Exosomes are small vesicles secreted by cells with a diameter of 30-150 nm, which contain biomolecules such as proteins and nucleotides derived from cells, and play an important role in c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/48A61K9/50A61K47/34A61P15/00C12N5/0775
CPCA61K9/5031A61K35/48A61P15/00C12N5/0668
Inventor 张冰晶鲁振宇洪敬欣
Owner 天津欣普赛尔生物医药科技有限公司
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