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Preparation containing autologous endometrium mesenchymal cell exosome

A kind of stem cell and endometrium technology, applied in the field of biological stem cells, can solve the problems of women's distress, long treatment time, difficult endometrial repair, etc., to promote the growth of epidermal cells, repair and reconstruction, and achieve endometrial structure and effect of function

Pending Publication Date: 2018-11-16
天津欣普赛尔生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The uterus is very important to a woman's life, but once the endometrium is damaged, it will cause a series of consequences. The existing endometrium is difficult to repair and the treatment time is long, which has brought great distress to many women

Method used

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  • Preparation containing autologous endometrium mesenchymal cell exosome
  • Preparation containing autologous endometrium mesenchymal cell exosome
  • Preparation containing autologous endometrium mesenchymal cell exosome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Cultivation of autologous endometrial mesenchymal stem cells: select mesenchymal stem cells from human endometrial cells from the cell bank for resuscitation, using phenol red-free DMEM / DF12 and a volume ratio of 10% fetal bovine serum Complete medium, put it in T75 cell culture flask at 37℃, 5% CO 2 Cultivate under the conditions;

[0029] (2) Preparation of mesenchymal stem cell exosomes: After the cell growth degree reaches 70%-80%, wash the cells 2-4 times with normal saline, and starve the cells for 12 hours with compound electrolyte injection, and wait until the cell growth degree reaches After 90%, the cell culture supernatant is collected, and the exosomes of mesenchymal stem cells are extracted with the exosome extraction kit, and human serum albumin is added according to the mass-volume ratio of 2%, and the mixture is evenly mixed before use;

[0030] (3) Preparation of matrix solution: according to the ratio, dissolve medicinal human albumin in compound electr...

Embodiment 2

[0033] (1) Cultivation of autologous endometrial mesenchymal stem cells: select mesenchymal stem cells from human endometrial cells from the cell bank for resuscitation, using phenol red-free DMEM / DF12 and a volume ratio of 10% fetal bovine serum Complete medium, put it in T75 cell culture flask at 37℃, 5% CO 2 Cultivate under the conditions;

[0034] (2) Preparation of mesenchymal stem cell exosomes: After the cell growth degree reaches 70%-80%, wash the cells 2-4 times with normal saline, and starve the cells for 16 hours with compound electrolyte injection, and wait until the cell growth degree reaches After 90%, the cell culture supernatant was collected, and the exosomes of mesenchymal stem cells were extracted with the exosome extraction kit, and human serum albumin was added according to the mass-volume ratio of 3%, and the mixture was evenly mixed for use;

[0035] (3) Preparation of matrix solution: According to the ratio, dissolve medicinal human albumin in 3% by weight a...

Embodiment 3

[0038] (1) Cultivation of autologous endometrial mesenchymal stem cells: select mesenchymal stem cells from human endometrial cells from the cell bank for resuscitation, using phenol red-free DMEM / DF12 and a volume ratio of 10% fetal bovine serum Complete medium, put it in T75 cell culture flask at 37℃, 5% CO 2 Cultivate under the conditions;

[0039] (2) Preparation of mesenchymal stem cell exosomes: After the cell growth degree reaches 70%-80%, wash the cells 2-4 times with normal saline, and starve the cells for 24 hours with compound electrolyte injection, and wait until the cell growth degree reaches After 90%, the cell culture supernatant is collected, and the exosomes of mesenchymal stem cells are extracted with the exosome extraction kit, and human serum albumin is added according to the mass-volume ratio of 4%, and the mixture is evenly mixed before use;

[0040] (3) Preparation of matrix solution: according to the ratio, dissolve medicinal human albumin in compound electr...

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Abstract

The invention provides a preparation containing endometrium mesenchymal cell exosome, and relates to the technical field of biological stem cells. The preparation comprises, by volume, 5 parts of endometrium mesenchymal cell exosome and 95 parts of matrix solution. The matrix solution comprises 95-99 parts of multiple electrolytes injection liquid and 1-5 parts of medicinal human blood albumin, and endometrium mesenchymal cell exosome is derived from starvation culture of autologous endometrium mesenchymal cells, and the time of the starvation culture is 12-24 hours. The autologous endometriummesenchymal cells are extracted from the mesenchymal stem cells in a human endometrium and cultured by using a normal culture method. The culture time is 48-72 hours. The invention provides a medicine which is safe, effective and convenient to use for treating diseases such as endometrial damage and the like.

Description

Technical field [0001] The invention relates to the technical field of biological stem cells, in particular to a preparation containing autologous endometrial mesenchymal stem cell exosomes. Background technique [0002] The uterus is very important to a woman's life, but once the endometrium is damaged, it will cause a series of consequences. The existing endometrium is difficult to repair and the treatment time is long, which brings great troubles to many women. [0003] Mesenchymal Stem Cells (MSC) Mesenchymal Stem Cells are pluripotent stem cells, which have multiple functions such as multidirectional differentiation, hematopoietic support and promotion of stem cell implantation, and immune regulation. They are mainly found in the endometrium, fat and bone marrow. in. [0004] Therefore, how to enable mesenchymal stem cells to be used to treat endometrial injury has become an urgent problem for those skilled in the art. Summary of the invention [0005] The technical problem to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P15/00C12N5/0775
CPCA61K9/0019A61K35/28A61K47/42A61P15/00C12N5/0668
Inventor 张冰晶鲁振宇洪敬欣
Owner 天津欣普赛尔生物医药科技有限公司
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