A new way to rapidly rebuild skin and hair follicles

Abstract

The invention discloses a new method and a model for rapidly rebuilding skin hair follicles, comprising the following steps: (1) cultivating full-thickness back skin tissue of mice or adult mice, and preparing homogenate tissue; (2) making the homogenate Transfer the tissue to a fiber membrane or silica gel membrane with air holes, and spread it evenly, at 37 ° C, containing 5% CO 2 (3) The membrane with the homogenized skin tissue cultivated in step (2) was bonded to the skin wound on the back of the nude mouse, with the homogenized tissue facing down and the membrane covering the top, Culture for more than 3 weeks. In the present invention, the reconstructed skin and hair follicle structure with hair can be obtained by culturing for 3 weeks. The invention is simple and fast in operation, can effectively rebuild skin hair follicles, and can enlarge and rebuild the skin area, and can rebuild skin tissues derived from newborn rats and adult rats. It will provide a basis for the application, scientific research and translational research of establishing reconstructed skin animal models in the future. This method is easy to popularize and use.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a new method for rapidly rebuilding skin and hair follicles. Background technique [0002] The skin is the largest organ and has the function of protecting the body's barrier. However, many skin wounds cannot recover by themselves, and may even cause infection and lead to death. The repair and regeneration of skin has always been a major problem that plagues human beings. Hair follicles are skin appendages composed of a variety of cells and are an important part of the skin with a characteristic cyclical cycle. An excellent hair follicle experimental model plays an important role in the study of hair regeneration, pigment formation, and wound healing. Studies have found that severe skin defect wounds may mainly rely on the proliferation of residual appendages, especially hair follicle cells, to heal. An average of 5 hair follicles can be cultured to form a 1cm 2 In addi...

AI Technical Summary

Problems solved by technology

[0004] At present, the reconstruction of hair follicles is mostly done through hair follicle stem cells. Obtaining hair follicle stem cells first requires separating the epidermis from the dermis, pulling out the hair follicles from the dermis, peeling off the hair follicle stem cell area under a dissecting microscope, and enzymatically digesting the separated cells. The entire operation process is time-consuming and laborious. The number of isolated cells is limited; there are also studies using mouse skin epidermal and dermal cells co-transplantation to induce hair follicle regeneration experiments, but these cells need to be isolated from the skin and cultured separately, and then transplanted into nude mice according to a 1:1 mixture, cultured for 6 The hair follicle structure can only be observed after a week, but the hair follicle structure cannot grow hair. At the same time, the method takes a long time to operate and the cell separation is cumbersome

In addition, in the current study, the hair follicle reconstruction method requires the use of freshly isolated neonatal mouse skin cells, but the source of neonatal mice is limited, and adult mice are more widely used in basic research and clinical research, and there are many sources, but Skin tissue derived from adult mouse hair follicles reconstructs a point where hair follicles have been difficult to break through

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