Biochip and chip control method

Abstract

The invention discloses a biochip and a chip control method. The biochip includes a chip substrate, and a quantitative sampling device, a plurality of reagent chambers and a reactor which are on arranged on the chip substrate; every reagent chamber is provided with a pipeline communicated with the reactor, and every pipeline is provided with a normally closed micro-valve; and the quantitative sampling device is provided with a sample outlet, and the sample outlet is communicated with the reactor. The biochip combines all the advantages of a large automatic analyzer and a biochip, strictly realizes the analysis precision of the traditional automatic analyzer, and even is better than the traditional automatic analyzer in some functions.

Description

technical field [0001] The invention relates to a biological chip and a chip control method, belonging to the field of biological chips. Background technique [0002] There are currently two types of existing technologies on the market: traditional large-scale automated analytical instrument detection technology and serial biochip technology. [0003] Such as figure 1 and 2 As shown, the traditional large-scale automatic analysis instrument adopts parallel technology, and the advantage of this technology is that it can achieve high-precision quantitative analysis and detection. The characteristic of the parallel technology is that each reagent is stored separately. According to the needs of the detection reaction, the reagents enter the same reactor sequentially through independent liquid channels to realize the analysis and detection of samples. [0004] The advantages of traditional large-scale automated analytical instrument detection technology are: [0005] 1. Sampl...

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Problems solved by technology

[0012] 1. Complex structure and inconvenient maintenance

[0013] 2. There are many liquid paths and waste liquid discharge

[0014] 3. The sample needle needs to be cleaned and other operations, which consume a large amount of reagents

[0015] 4. Portable and on-site instant diagnosis cannot be achieved

[0018] However, because this serial microfluidic chip technology is based on the above-mentioned inherent characteristics, it cannot achieve the precision of large-scale automated analytical instruments, and has the following disadvantages:

[0019] 1. This method cannot avoid cross-contamination

[0020] 2. This method cannot achieve accurate sample addition of reagent volume

[0021] 3. The mixing process is irreversible, and there is a risk of inconsistent mixing degree

[0022] 4. The cleaning process is irreversible, and there is a risk of incomplete cleaning

However, this patent uses ice as the spool, so it cannot be used for storage of reagents, and there is no spool under normal conditions; further, ice is easy to react with reagents, and ice is too large as a spool (it cannot be used as a valve core). to the micron level), precise control cannot be achieved. In addition, if the patent is used on a microfluidic chip, then the chip needs to be implanted with circuits, which will further increase the difficulty of chip manufacturing and reduce its reliability.

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