Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of streptomyces griseus and its fermentative production of bafilomycin a1

A technology of streptomyces and tan, applied in the direction of fermentation, microbial-based methods, bacteria, etc., can solve the problem of low fermentation titer, achieve the effect of increasing titer and meeting the needs of industrialization

Active Publication Date: 2019-11-08
FUJIAN INST OF MICROBIOLOGY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For this reason, the technical problem to be solved by the present invention is to provide a kind of Streptomyces griseus that produces bafilomycin A1, to solve the problem that the fermentation titer of bafilomycin A1 compound is low in the prior art;

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of streptomyces griseus and its fermentative production of bafilomycin a1
  • A kind of streptomyces griseus and its fermentative production of bafilomycin a1
  • A kind of streptomyces griseus and its fermentative production of bafilomycin a1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 strain source

[0027] In August 2012, our laboratory obtained several strains of actinomycetes in the sediments of the East China Sea through isolation, culture, fermentation, and activity determination. After preliminary determination, one strain of Streptomyces had strong antibacterial activity against Aspergillus niger.

[0028] The compound Ba0115 was obtained through natural separation and purification, fermentation and post-extraction process of the strain, which was identified as homogeneous to bafilomycin A1 by mass spectrometry and NMR. However, the fermentative production of bafilomycin A1 by the original strain has a low titer. In this embodiment, this strain is used as the starting strain for mutagenesis.

[0029] Preparation of spore suspension: inoculate the starting strain on the ISP2 slope at 28°C for about 10 days, take the fresh slope and add 10 mL of sterile saline, gently scrape it off with an inoculation spatula, pour it into a sterile sh...

Embodiment 2

[0035] Morphological and culture characteristics of embodiment 2 Streptomyces FIM-Ba150115

[0036] After identification, Streptomyces FIM-Ba150115 described in this example was positive by Gram staining. After growing for 7 days on the culture mediums such as Gaoshi Synthetic No. 1 Agar and ISP2, the hyphae in the base were well developed, without septa, and without Fractured, aerial hyphae grow well, multi-branched, spore filaments are straight or flexible.

[0037] Seven kinds of media including ISP1, ISP2, ISP3, and ISP4 were used respectively, and after culturing at 30°C for 14 days, the color and pigment of mycelium were observed. The results are shown in Table 2 below.

[0038] Table 2 Culture characteristics of FIM-Ba150115

[0039] culture medium growing situation aerial hyphae base hyphae Soluble pigment ISP1 ++ gray grey none ISP2 +++ taupe taupe none ISP3 + Ash poor none ISP4 + light gray poor none ...

Embodiment 3

[0044] The detection of bafilomycin A1 in the fermented liquid of embodiment 3

[0045] In the following examples of the present invention, the detection of the content of bafilomycin A1 in the fermentation broth is carried out according to the HPLC method in the prior art.

[0046] Fermentation sample preparation: Take 10 mL of the fermentation liquid of the strain, add 20 mL of methanol and mix, oscillate and mix for 20 min, centrifuge, set aside, and use HPLC method to detect bafilomycin A1.

[0047] High performance liquid chromatography analysis conditions Chromatographic column detection conditions include:

[0048] Ultimate LP-C18 analytical column (5μm, 4.6×250mm);

[0049] Mobile phase: methanol-water solution with a volume ratio of 85:15;

[0050] Flow rate: 1.0mL / min;

[0051] Injection volume: 10μL;

[0052] Detection wavelength: 247nm;

[0053] Use time: 30min;

[0054] The content was calculated using the peak area.

[0055] Scan the bafilomycin A1 referen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of microbial fermentation, and in particular relates to a Streptomyces griseus producing bafilomycin A1, and further discloses the application of the fermentation to produce bafilomycin A1. The present invention obtains a high-yield bafliomycins compound Streptomyces griseus by means of strain screening and mutation breeding screening. ‑The titer of bafliomycin A1 produced by fermentation of Ba150115 is as high as 615.87mg / L, which greatly facilitates the extraction and purification of bafliomycin A1 and can meet the needs of industrialization.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a Streptomyces griseus producing bafilomycin A1, and further discloses the application of the fermentation to produce bafilomycin A1. Background technique [0002] Bafliomycins are a class of polyene macrolide antibiotics with a 16-membered ring skeleton produced by the fermentation of actinomycetes. The chemical structure is very special. Most of these compounds contain a six-membered ring hemicondensation Aldehyde structure. The reported bafilomycins compounds include bafilomycin A1, A2, B1, B2, C1, C2, D, E, F, K and L, etc. Bafilomycins have various biological activities such as antibacterial, antifungal, insecticidal, antitumor and immunosuppressive, and can also be used to treat osteoporosis and deafness. And existing studies have shown that Bafilomycins series compounds, especially bafilomycinA1, have specific autophagy inhibitory effect and be...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P17/16C12R1/465
CPCC12P17/162C12N1/205C12R2001/465
Inventor 周剑江红
Owner FUJIAN INST OF MICROBIOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com