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A Headspace Solid Phase Microextraction-Gas Chromatography Detection and Analysis Method for the Essential Oil Components of Seabuckthorn

A solid-phase microextraction head and gas chromatography-mass spectrometry technology, which can be applied in analysis materials, measuring devices, material separation, etc., can solve the problem of less volatile oil of sea buckthorn, and achieve the effects of accurate qualitative, reliable results and high degree of automation

Active Publication Date: 2021-10-29
INSPECTION AND QUARANTINE TECHNOLOGY CENTER ZHONGSHAN ENTRY EXIT INSPECTION AND QUARANTINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, people's research on seabuckthorn mainly focuses on chemical components such as flavonoids, amino acids, fatty acids, etc. There are few reports on seabuckthorn volatile oil

Method used

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  • A Headspace Solid Phase Microextraction-Gas Chromatography Detection and Analysis Method for the Essential Oil Components of Seabuckthorn
  • A Headspace Solid Phase Microextraction-Gas Chromatography Detection and Analysis Method for the Essential Oil Components of Seabuckthorn
  • A Headspace Solid Phase Microextraction-Gas Chromatography Detection and Analysis Method for the Essential Oil Components of Seabuckthorn

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, detection and analysis of seabuckthorn volatile oil components

[0025] 1 Materials and reagents

[0026] Sodium chloride, anhydrous sodium sulfate, and n-hexane were of analytical grade and purchased from Guangzhou Chemical Reagent Factory, China; n-alkane mixed standard (C7-C40) was purchased from O2si Company, USA.

[0027] Seabuckthorn dried medicinal material (origin: Inner Mongolia, batch number: 180201), with a moisture content of 7.8% and a total ash content of 3.8%, was purchased from Nanguo Yaodu Traditional Chinese Medicine Pieces Co., Ltd., Hunan Province.

[0028] 2 Instruments and equipment

[0029] TSQ 8000 gas chromatography-triple quadrupole tandem mass spectrometer: equipped with electron ionization source and Xcalibur data processing system, TRIPLUS RSH autosampler, 20mL headspace sample bottle, solid phase microextraction automatic sampling handle, American Thermo company; 50 / 30 μm DVB / CAR / PDMS solid-phase microextraction head, Supel...

Embodiment 2

[0048] Embodiment 2, the screening of headspace solid-phase microextraction condition

[0049] Since the extraction effect of the headspace-solid phase microextraction method is affected by conditions such as extraction time, extraction temperature, desorption time, and equilibrium time, the present invention compares the The effects of different extraction time, extraction temperature, desorption time, and equilibrium time on the extraction effect of seabuckthorn volatile substances, and determine the optimization parameters.

[0050] The experimental method is carried out according to Example 1, wherein the extraction time, extraction temperature, desorption time, and equilibrium time are selected according to Table 2, and the results of different extraction times, extraction temperatures, desorption time, and equilibrium time on the extraction effect of seabuckthorn volatile substances are shown in Table 2 .

[0051] Table 2 Effects of different factors on the extraction e...

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Abstract

The invention provides a headspace solid-phase microextraction-gas chromatography detection and analysis method for the volatile oil components of seabuckthorn. The present invention analyzes seabuckthorn by the method of headspace solid-phase microextraction-gas chromatography, detects 106 peaks in total, and identifies 76 components thereof. After comparison, it is found that the seabuckthorn volatile oil components identified by the present invention are far more than those reported in the prior art, indicating that the detection method and qualitative analysis means of the present invention have significant advantages. Moreover, the method of the present invention has high degree of automation, simple operation, more accurate qualitative and more reliable results, and can also be applied to the detection and analysis of volatile components of other medicinal and edible homologous plants, so as to control the quality of medicinal and edible homologous plants and promote the development of medicinal and edible homologous plants. The development of homologous industries provides scientific support.

Description

technical field [0001] The invention relates to a headspace solid-phase microextraction-mass chromatography detection and analysis method for volatile oil components of seabuckthorn. Background technique [0002] Seabuckthorn (Hippophae rhamnoides Linn.), also known as sour thorn, is the dry and mature fruit of the plant of the genus Hippophae rhamnoides. Phlegm, invigorating the spleen and eliminating food, etc., can be used to treat spleen deficiency, lack of food, abdominal pain due to food accumulation, cough with excessive phlegm, chest pain and heartache, blood stasis, amenorrhea, tumbling and bruising and other diseases. Seabuckthorn has strong adaptability, can tolerate drought, cold, salt and alkali, and wind and sand, and has a positive effect on the improvement of the ecological environment. It is mainly planted in the northwest, southwest, and northeast regions of China. Seabuckthorn is rich in nutrients and contains a variety of biologically active ingredients,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 李蓉张鹏云卢俊文杨志方
Owner INSPECTION AND QUARANTINE TECHNOLOGY CENTER ZHONGSHAN ENTRY EXIT INSPECTION AND QUARANTINE
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