A kind of β2 microglobulin assay kit and its detection method
A technology of β2 microglobulin and kits, applied in measuring devices, color/spectral characteristic measurement, instruments, etc., can solve problems such as poor repeatability and cumbersome operation, and achieve low cost, good repeatability, and both detection sensitivity and linearity range effect
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Embodiment 1
[0033] Example 1 Preparation of Latex Particle Anti-Human β2-MG Antibody Conjugated Emulsion
[0034] The preparation method of latex particle anti-human β2-MG antibody binding emulsion comprises the following steps:
[0035] 1) Take 2mL of carboxylated latex microspheres with a particle size of 108nm and a solid content of 5%, add MES buffer to 5mL;
[0036] 2) Add 1mL of 22g / L N-hydroxysuccinimide and 1mL of 8.5g / L 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride for activation, stir After uniformity, the first incubation was carried out at a temperature of 37°C for 20 minutes to obtain activated polystyrene latex microsphere particles;
[0037] 3) Add 3mL of MES buffer, stir evenly at room temperature, add anti-human β2-MG monoclonal antibody at a ratio of 0.035:1 mass ratio of anti-human β2-MG monoclonal antibody to the polystyrene latex microsphere particles, and carry out For the second incubation, the temperature is 37°C and the time is 4h;
[0038] 4) Aft...
Embodiment 2
[0039] Example 2 Preparation of Latex Particle Anti-Human β2-MG Antibody Conjugated Emulsion
[0040] The preparation method of latex particle anti-human β2-MG antibody binding emulsion comprises the following steps:
[0041] 1) Take 1.5 mL of carboxylated latex microspheres with a particle size of 108 nm and a solid content of 5%, and add MES buffer to 5 mL;
[0042] 2) Add 2mL of 22g / L N-hydroxysuccinimide and 2mL of 8.5g / L 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride for activation, stir well Afterwards, the first incubation was carried out at a temperature of 37°C for 20 minutes to obtain activated polystyrene latex microsphere particles;
[0043] 3) Add 5 mL of MES buffer solution, stir evenly at room temperature, add anti-human β2-MG monoclonal antibody at a ratio of 0.08:1 mass ratio of anti-human β2-MG monoclonal antibody to the polystyrene latex microsphere particles, and carry out For the second incubation, the temperature is 37°C and the time is 4h;...
Embodiment 3
[0045] Example three β2 microglobulin assay kit and detection method
[0046] The β2 microglobulin assay kit includes reagent R1 and reagent R2; the volume ratio of reagent R1 to reagent R2 is 4:1.
[0047] R1: Phosphate buffer 80mmoL / L, NaCl 10g / L, polyethylene glycol 6000 15g / L, Proclinc 3001.5g / L;
[0048] R2: MOPS buffer 100mmoL / L, trehalose 80g / L, latex particle anti-human β2-MG antibody-conjugated emulsion 0.06g / L prepared in Example 1, NaCl 14.5g / L, Tween-20 10g / L, bovine Serum albumin (BSA) 20g / L, betaine 10g / L, Proclin 300 1.5g / L.
[0049] The detection method of the β2 microglobulin assay kit includes the following steps: separate the serum sample, add reagent R1 to the serum sample, mix well, incubate for 4 minutes, add R2 and mix well and incubate for 30 seconds, then detect the absorbance value A1 at a wavelength of 600nm After 5 minutes, the absorbance value A2 was detected at a wavelength of 600nm, a standard curve was established based on the serum standard d...
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