Nested PCR-based primer group for detecting Salmonella spp., application of primer group, kit containing primer group, and detection method using kit

Abstract

The invention belongs to the technical field of bacteria detection, and particularly relates to a nested PCR-based primer group for detecting Salmonella spp., application of the primer group, a kit containing the primer group and a detection method using the kit. The nested PCR-based primer group for detecting Salmonella spp. comprise two pairs of primers, wherein outer primers are F1 and R1, inner primers are F2 and R2, the nucleotide sequence of F1 is as shown in SEQ ID NO.1, the nucleotide sequence of R1 is as shown in SEQ ID NO.2, the nucleotide sequence of F2 is as shown in SEQ ID NO.3, and the nucleotide sequence of R2 is as shown in SEQ ID NO.4. The invention has the advantages that the nested PCR-based primer group for detecting Salmonella spp. is good in specificity, the kit prepared by using the primer group has good specificity and high sensitivity, and has a lowest detectable limit of 10<2> CFU/mL for Salmonella spp., and the detection method using the primer group is simple in operation and low in cost for detecting Salmonella spp., and can be applied to accurate sensitive detection of clinical, food and complex samples.

Description

technical field [0001] The invention belongs to the technical field of bacterial detection, and in particular relates to a nested PCR-based primer set for detecting Salmonella, and at the same time relates to the use of the primer set, a kit prepared by using the primer set, and the kit for detecting intestinal serotype Salmonella detection method. Background technique [0002] Salmonella (Salmonella spp.) is a large group of Gram-negative bacilli (G-) that parasitize in the intestines of humans and animals, and is the most common zoonotic pathogenic bacteria in the Enterobacteriaceae family. Currently there are more than 2,500 known serotypes of Salmonella, and except for less than 10 rare serotypes belonging to Salmonella Bongor, the rest of the serotypes belong to Salmonella enterica, so almost all Salmonella can cause serious food poisoning incidents. Invasion is the main key factor for Salmonella to enter the body and cause disease. Virulent Salmonella can invade the e...

AI Technical Summary

Problems solved by technology

[0003] At present, the detection of Salmonella is mainly based on traditional methods such as bacterial isolation, serotype experiments, and biochemical identification, which usually require a detection cycle of 5-7 days, and there are defects such as cumbersome operations, low sensitivity, and low accuracy, which cannot meet Timely and effective quality monitoring and disease control needs

At the same time, there are detection methods with higher sensitivity on the market, such as conventional PCR detection technology, which i

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