Preparation and application of pesticide rapid test card

A rapid test card and pesticide technology, applied in the field of preparation and application of pesticide quick test card, can solve the problems of complex production process and high cost

Active Publication Date: 2019-01-22
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although test strips have been used to a certain extent, the existing methods are relatively complex and costly to make, and the mechanism of action of the above test strips is fundamentally different from that of pesticide small molecule detection.

Method used

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  • Preparation and application of pesticide rapid test card
  • Preparation and application of pesticide rapid test card
  • Preparation and application of pesticide rapid test card

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation process of composite catalytic material is characterized in that the method comprises the steps:

[0038] (1) Add a certain amount of sodium borohydride to the mixed solution containing BSA and divalent cobalt ions, and obtain Co by the reduction of sodium borohydride and the oxidation of oxygen 3 o 4 NPs, centrifuged and dried to obtain a solid;

[0039] (2) A certain amount of Co 3 o 4 NPs were added to 0.2 mM BOC solution to protect Co 3 o 4 Amino groups on NPs were washed and dried after standing for 2 hours;

[0040] (3) Co obtained in step (2) 3 o 4 NPs were added to the mixed solution of EDC and NHS with a mass concentration of 2% to activate Co 3 o 4 Carboxyl groups on NPs, let stand for 5 hours, wash with water, and dry;

[0041] (4) Co 3 o 4 NPs are added to the mixture of AchE and ChOx, and the amino groups on AchE and ChOx will interact with Co 3 o 4 The carboxyl group on NPs undergoes an amide reaction to immobilize them o...

Embodiment 2

[0043] The preparation process of the quick test card ( figure 1 ):

[0044] (1) Drop the BSA solution with a mass concentration of 1% on the cellulose membrane, let it stand for 1 hour and dry it at room temperature;

[0045] (2) Drop EDC and NHS with a mass concentration of 2% on the cellulose membrane modified with BSA described in step (1) (nitrocellulose membrane with good water absorption) to activate the carboxyl group of BSA, and let stand 5 hours, washed with water and dried at room temperature;

[0046] (3) Evenly drop-coat the solution of the composite catalytic material onto the cellulose membrane obtained in step (2), the amino group on the composite catalytic material will undergo an amide reaction with the carboxyl group on the BSA to fix it on the cellulose membrane, 4 overnight at ℃, washed with water and dried;

[0047] (4) Drop-coat the organic chromogen solution onto the cellulose membrane modified with the composite catalytic material described in step...

Embodiment 3

[0051] Feasibility of quick test card detection:

[0052] Catalyzed reaction system (a) Cut the color chart into pieces and put it in acetylcholine (1 mmol / L) solution (pH=5.0). After reacting for 5 minutes, take the reaction solution for full-wavelength scanning. Do another set of control experiments (b) Cut the colorimetric card into pieces and put them in the buffer solution (pH=5.0), react for 5 minutes and take the reaction solution for full-wavelength scanning.

[0053] Such as image 3 As shown, the catalytic reaction system (a) has an obvious ultraviolet absorption peak, and the catalytic reaction system (b) has no ultraviolet absorption peak, which proves that the catalytic reaction can only be carried out under the condition of the presence of acetylcholine.

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Abstract

The invention relates to the fields of nano materials, catalysis and analysis chemistry, and specifically relates to preparation and application of a pesticide rapid test card. According to the method, cobalt oxide nanospheres with peroxidase-like activity, acetylcholinesterase, choline oxidase and a color developing agent are used to modify filter paper so as to obtain a rapid test card for rapiddetection. The rapid test card detects acetylcholine and organic phosphorus pesticide easily and rapidly. The novel colorimetric pesticide detection method is highly practical, thereby having a broadapplication prospect in fields like food detection, analysis chemistry, environment engineering and agriculture.

Description

technical field [0001] The invention relates to the fields of nanomaterials, catalysis and analytical chemistry, and specifically includes the preparation and application of a pesticide quick test card. technical background [0002] With the development of agricultural technology, pesticides have been widely used. Pesticide residues have become an important hidden danger that threatens human food safety. Therefore, the detection of pesticides has become particularly important and has attracted great attention. There have been many articles on pesticide detection, for example, Jung et al in "M13 bacteriophage / silver nanowire surface-enhanced raman scattering sensor for sensitive and selective pesticide detection" using M13 bacteriophage functionalized silver nanowires to be sensitive and selective by Raman detection of pesticides ( ACS Applied Materials & Interfaces 2018, October, 10388-10397). In "Two-dimensional 1T-phase transition metal dichalcogenides as nanocarrier...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 韩磊张海姣李峰
Owner QINGDAO AGRI UNIV
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