Method for detecting organic phosphorus pesticide residues in wine

A detection method, wine technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of large amount of toxic and harmful chemical reagents, cumbersome pretreatment methods, and large environmental pollution, and achieve low cost, simple and fast method, and high sensitivity high effect

Inactive Publication Date: 2019-01-22
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the pretreatment methods of alcoholic pesticide residues at home and abroad mainly include solid-phase extraction and solid-phase microextraction, etc., but these pretreatment

Method used

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  • Method for detecting organic phosphorus pesticide residues in wine
  • Method for detecting organic phosphorus pesticide residues in wine
  • Method for detecting organic phosphorus pesticide residues in wine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for detecting organophosphorus pesticide residues in wine, taking dry red wine as an example, consists of the following steps:

[0037] A. Draw 10mL of the wine sample to be tested and place it in a centrifuge tube;

[0038] B. Add 10-30mL of acetonitrile to the centrifuge tube in step A, then place the centrifuge tube in a liquid mixer, and extract homogeneously at 2000-3000r / min for 1-2min;

[0039] C. Add 2-10g of sodium chloride to the homogenized centrifuge tube in step B, and extract homogeneously for 1-2min at 2000-3000r / min;

[0040] D. Place the centrifuge tube in step C in a centrifuge and centrifuge at 5500-6500r / min for 2-4min;

[0041] E. Take 5-15 mL of the supernatant after centrifugation in step D, place it on a rotary evaporator, and rotate it to dryness at a temperature of 35-45 ° C;

[0042]F. Add n-hexane acetone solution to the dried supernatant in step E and set the volume to 1 mL. The volume ratio of the n-hexane acetone to the dried su...

Embodiment 2

[0047] A method for detecting organophosphorus pesticide residues in wine, taking dry white wine as an example, the pretreatment steps for the determination of organophosphorus pesticide residues in dry white wine samples are the same as in Example 1. The detection conditions of gas chromatography mass spectrometry are the same as in Example 1. The above test results are shown in Table 3.

[0048] Table 3 Detection results and recoveries of organophosphorus pesticide residues in dry white wine samples

[0049]

[0050] According to the detection results, it can be seen that the relative deviation of the detection results of the present invention is small, and the recovery rate is high, which is obviously better than the existing common chromatography mass spectrometry detection.

Embodiment 3

[0052] A method for detecting organophosphorus pesticide residues in wine. Taking sparkling wine as an example, the pretreatment steps for the determination of organophosphorus pesticide residues in sparkling wine samples are the same as in Example 1. The detection conditions of gas chromatography mass spectrometry are the same as in Example 1. The above test results are shown in Table 4.

[0053] Table 4 Detection results and recoveries of organophosphorus pesticide residues in sparkling wine samples

[0054]

[0055] According to the detection results, it can be seen that the relative deviation of the detection results of the present invention is small, and the recovery rate is high, which is obviously better than the existing common chromatography mass spectrometry detection.

[0056] like figure 1 As shown, the total ion current of the mixed standard solution of 9 kinds of organophosphorus pesticide residues, dichlorvos, methimen, phorate, methyl parathion, chlorpyrif...

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Abstract

The invention relates to a method for determining pesticide residues, in particular to a method for detecting organic phosphorus pesticide residues in wine. A sample pretreatment method for liquid-liquid direct extraction is established, in the process of sample pretreatment, the method is simple and rapid, reagents are saved, the cost is low, environmental protection is facilitated, and sensitivity and accuracy both meet the requirements of pesticide residue analysis. The method can be used for performing the sample pretreatment on daily detection and analysis of nine kinds of organic phosphorus pesticide residues of dichlorvos, ethoprophos, phorate, methyl parathion, chlorpyrifos, fenthion, methidathion, triazophos and phosalone in wine, the nine kinds of organic phosphorus pesticide residues after the sample pretreatment are detected by a gas chromatography-mass spectrometry, the detection results are accurate, the interference was small, and high social and economic benefits are achieved.

Description

technical field [0001] The invention relates to a method for detecting pesticide residues, in particular to a method for detecting organophosphorus pesticide residues in wine. Background technique [0002] Wine is an industry with special charm in the history of social, economic and cultural development. At present, nearly 90 countries in the world produce wine. Wine is an international beverage rich in nutrition, and its status among commodities is increasing day by day. In recent years, the total output of wine in the world has basically remained at about 27 million tons, and the average annual consumption is about 22 million tons. The market is very active. [0003] Wine is a beverage fermented from fresh grapes or grape juice, which contains many nutrients, such as amino acids, mineral elements (including trace elements) and vitamins necessary for the human body, as well as sitosterol, polyphenols and other ingredients, so it is not only nutritious Drinking in moderati...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 党富民雷用东向晓黎罗瑞峰孙凤霞姬勇谢勇王静唐宗贵杨涛逯霞
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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