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Preparation method for piglet enterotoxigenic escherichia coil-resistant egg yolk antibody

A technology of Escherichia coli and egg yolk antibody is applied in the preparation of anti-piglet toxin-producing Escherichia coli egg yolk antibody and in the field of anti-piglet toxin-producing Escherichia coli antibody, which can solve the problem of lack of good medicine for treating piglet Escherichia coli diarrhea, etc. The effect of novel veterinary biological drug development, reducing economic losses and reducing the incidence of diarrhea

Active Publication Date: 2019-02-05
青岛今墨堂生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This antibody has practical value for the emergency treatment of diarrhea caused by Escherichia coli in piglets, and this application solves the technical problem that there is no good drug to treat diarrhea caused by Escherichia coli in piglets in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A preparation method for anti-pig yolk antibody of toxin-producing Escherichia coli comprises the following steps in sequence:

[0036] 1) SPF laying hens aged 20 to 30 weeks were immunized with piglet Escherichia coli K88 and K99 bivalent genetically engineered live vaccines;

[0037]2) SPF laying hens were immunized with intestinal isolates from piglets with Escherichia coli diarrhea, enterotoxigenic Escherichia coli, enterotoxin and adhesive pili protein mixed immunogen: inject piglets with colibacillosis K88 5 days before the first immunization , K99 bivalent genetic engineering live vaccine and mixed immunogen for immune stimulation, inject 1ml at the first immunization; carry out the second immunization after 2 weeks, inject piglet colibacillosis K88, K99 bivalent genetic engineering live vaccine and mixed immunogen respectively 1ml; carry out the third immunization every other 2 weeks, and inject piglet colibacillosis K88, K99 bivalent genetic engineering live va...

Embodiment 2

[0040] The composition of the traditional Chinese medicine composition is: 20 kg of raw astragalus, 20 kg of silver flower, 15 kg of Bupleurum, 15 kg of scutellaria, 20 kg of banlangen, 20 kg of Guanzhong, 15 kg of atractylodes, 20 kg of raw coix seed, 15 kg of Fangfeng, 10 kg of raw licorice and 15 kg of Huoxiang, crushed through a 120-mesh sieve.

[0041] Others were the same as in Example 1 to obtain No. 2 yolk antibody purified product.

Embodiment 3

[0043] The composition of the traditional Chinese medicine composition is: 15 kg of raw astragalus, 15 kg of silver flower, 10 kg of Bupleurum, 10 kg of scutellaria, 15 kg of banlangen, 15 kg of Guanzhong, 10 kg of atractylodes, 15 kg of raw coix seed, 10 kg of windproof, Raw licorice 5 kg, Huoxiang 10 kg, crushed through a 110-mesh sieve.

[0044] Others were the same as in Example 1 to obtain No. 3 yolk antibody purified product.

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PUM

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Abstract

The invention provides a preparation method for a piglet enterotoxigenic escherichia coil-resistant egg yolk antibody, and belongs to the technical field of antibody preparation. The preparation method for the piglet enterotoxigenic escherichia coil-resistant egg yolk antibody adopts an existing piglet enterotoxigenic escherichia coil gene engineering inactivated vaccine and adopts piglet escherichia coli diarrhea diseased pig intestinal tract isolate enterotoxin and adhesive pilin to perform muscle immune on an SPF laying hen, in an immunologic process, Enterococcus faecium Anp01 CCTCC M 200919 is fed and a traditional Chinese medicine composition is smashed for feeding serving as an immunopotentiator, eggs of 2-6 weeks of immune infectious enteritis are collectd, an egg yolk aqueous extract solution is extracted, the egg yolk aqueous extract solution is purified by using a freeze-thaw method, so as to obtain the egg yolk antibody IgY. The antibody has a practical value to emergency treatment of diarrhea caused by piglet enterotoxigenic escherichia coil, and has important significance on reducing economic loss of pig industry and promoting development of a new veterinary biological drug.

Description

technical field [0001] The invention belongs to the technical field of antibody preparation, and relates to an anti-piglet toxigenic Escherichia coli antibody, in particular to a preparation method of an anti-piglet toxigenic Escherichia coli egg yolk antibody. Background technique [0002] Piglet diarrhea is one of the main problems perplexing large-scale pig production, which has brought huge economic losses to the pig industry. Pathogenic Escherichia coli is the most important pathogen causing diarrhea in newborn piglets and weaned piglets, among which enterotoxigenic escherichia coil (etec) is the most important pathogenic bacteria. The normal flora is destroyed or drug-resistant strains are produced, and the treatment effect is not good. The preparation method of the egg yolk antibody preparation for treating Escherichia coli diarrhea in piglets of the present application solves the technical problem that there is no good medicine for treating Escherichia coli diarrhea...

Claims

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Application Information

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IPC IPC(8): C07K16/12C07K16/02
CPCC07K16/02C07K16/1232C07K2317/11
Inventor 邵峰刘光辉
Owner 青岛今墨堂生物技术有限公司
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