Fermented carbonated honeysuckle particle and preparation method and application thereof

A honeysuckle and carbonization technology, which is applied to the field of fermented and carbonized honeysuckle particles and their preparation, can solve the problems of insignificant adsorption effect of harmful substances in smoke, poor coordination of cigarette aroma, inability to impart fragrance to cigarettes, etc. The effect of coke dual effect, good commercial application value and low cost

Active Publication Date: 2019-02-12
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the disadvantages of the existing plant particles, such as insignificant adsorption effect on smoke harmful substances, inability to directly add aroma to cigarettes, or poor coordination with cigarette aroma, etc., the present invention provides a fermented carbonized honeysuckle granule and its preparation method and application. By modifying the plant raw materials by using the fermenting and carbonization process of the tobacco Soil Dainella L4-6, it is possible to provide a product that can not only enhance the effect of cigarette harm reduction and tar reduction, reduce the irritation of cigarette smoke, but also enhance the effect of cigarette flavoring and enhance the quality of cigarettes. cigarette smoking quality

Method used

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  • Fermented carbonated honeysuckle particle and preparation method and application thereof
  • Fermented carbonated honeysuckle particle and preparation method and application thereof
  • Fermented carbonated honeysuckle particle and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]1. Isolation, cultivation and identification of Tobacco Solanum Daiia L4-6

[0040] 1.1 Isolation of Tobacco Soil Daeella L4-6

[0041] Tobacco soil samples were taken from the tobacco planting base in Kunming, Yunnan, sealed in plastic bags, and stored at 4°C for later use. Accurately weigh 10g of soil sample, add 90mL of sterile water, shake on a shaker at 30°C and 180rpm for 30min; dilute the concentration to 10 with sterile water -1 、10 -2 、10 -3 、10 -4 、10 -5 Take 0.2mL of the five concentrations of bacterial solution and spread it on the LB plate medium, set up three parallel experiments for each concentration of the bacterial solution, cultivate it at 30°C for 48 hours, and pick it from the plate of each soil sample For different single colonies, after purification on LB plate medium, 20% of the bacterial volume was added to glycerol, and stored in a -80°C refrigerator for later use.

[0042] The isolated microorganisms were respectively inoculated in LB liq...

Embodiment 2

[0058] 1. Cultivation of Tobacco Soil D. L4-6

[0059] (1) Test tube slant culture

[0060] Slope preservation medium is used, the medium is R2A agar medium, and the formula of the medium is: 0.5g of glucose, 0.5g of yeast extract, 0.5g of peptone, 0.5g of acid hydrolyzed casein, 0.5g of soluble starch, and 0.3g of sodium pyruvate , dipotassium hydrogen phosphate 0.3g, magnesium sulfate 0.05g, agar 15g, distilled water to 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minutes, place it on a slant, inoculate it with the strain L4-6 of Agrobacterium nicotianae, and cultivate it at 28°C for 1 week to obtain the strains in the test tube;

[0061] (2) Seed cultivation

[0062] Adopt seed medium, the formula of seed medium is: dextrin 120g, soybean powder 40g, yeast extract 2g, tryptophan 0.5g, β-alanine 5g, magnesium sulfate 0.5g, ammonium phosphate 0.2g, distilled water constant volume to 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minutes, pick pa...

Embodiment 3

[0073] 1. The culture method of Tobacco Soil Dainella L4-6 is the same as in Example 2.

[0074] 2. Fermentation of honeysuckle

[0075] Weigh 600g of honeysuckle with pre-balanced water content to 12%, spray 120mL of inoculum of Agrobacterium nicota L4-6, put the treated honeysuckle in a 22°C, 60% constant temperature and humidity box to ferment for 72h.

[0076] 3. Carbonization of fermented honeysuckle

[0077] Put the fermented honeysuckle into the carbonization furnace, gradually raise the temperature of the furnace to 80°C, and keep it for 30 minutes. After the honeysuckle fermentation sample is dried, quickly raise the temperature to 130°C, and keep it for 3 hours. After checking the degree of carbonization, turn off the heat source, and take out the fermentation carbonization The honeysuckle samples are ready for use.

[0078] 4. Preparation of fermented carbonized honeysuckle granules

[0079] Take fermented carbonized honeysuckle, detect its water content, make up...

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Abstract

The invention provides a fermented carbonated honeysuckle particle and a preparation method and application thereof. The fermented carbonated honeysuckle particle is prepared through the following steps that (1) a dyellatabacisoli L4-6 agent is prepared; (2) the dyellatabacisoli L4-6 agent is used for fermenting honeysuckle to obtain fermented honeysuckle; (3) the fermented honeysuckle is carbonated to obtain fermented carbonated honeysuckle; (4) fermented carbonated honeysuckle powder is placed on a fluidized bed to be dried, an adhesion agent is sprayed, the powder is adhered to form a particle, and the particle is continuously dried to obtain the fermented carbonated honeysuckle particle. The particle is fermented, so that special fragrance coordinating with cigarette smoke can be generated after the particle is applied to a cigarette, thereby not only enriching the cigarette fragrance, but also having the pharmacological effects of relieving sore throat, clearing heat, removing inflammation and the like; the particle is carbonated, so that the specific surface area of adsorption in the honeysuckle can be obviously increased, thereby enhancing the adsorption function of the cigarette smoke on harmful substances, and giving the plant particle with special burnt flavor.

Description

technical field [0001] The invention belongs to the technical field of cigarette materials, and in particular relates to fermented carbonized honeysuckle granules and a preparation method and application thereof. Background technique [0002] Low coking has become an inevitable trend in the development of the tobacco industry. Low coking brings about a reduction in the taste of cigarettes, and insufficient aroma has gradually become a prominent problem in the development of tobacco. How to overcome the key technologies that affect the aroma and aroma of tobacco leaves has become the core of improving the competitiveness of cigarette production. Adding tobacco flavors and fragrances to shredded cigarettes is a conventional and necessary technical means to add aroma, but the space for improving the quality of cigarettes is relatively limited. It has become a new development trend to improve the aroma of cigarettes and reduce the irritation of cigarette smoke. Among them, add...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24D3/14C01B32/05C12N1/20C12R1/01
CPCA24D3/14C12N1/20C01B32/05
Inventor 高莉段焰青夏建军李文均焦俊杜刚李先毅
Owner CHINA TOBACCO YUNNAN IND
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