Short lipopeptid with specific anti-propionibacterium acne effect and anti-inflammatory effect

A lipopeptide and acne technology, which can be applied to medical preparations containing active ingredients, peptides, hybrid peptides, etc., can solve the problems of secondary infection and poor specificity, and achieve low toxicity, low cost, and short amino acid sequence length. Effect

Active Publication Date: 2019-02-12
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Secondly, most of these peptides have broad-spectrum antibacterial activity, and their specificity is poor. When used, they may also kill beneficial bacteria in the human body, resulting in secondary infection

Method used

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  • Short lipopeptid with specific anti-propionibacterium acne effect and anti-inflammatory effect
  • Short lipopeptid with specific anti-propionibacterium acne effect and anti-inflammatory effect
  • Short lipopeptid with specific anti-propionibacterium acne effect and anti-inflammatory effect

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Embodiment 1

[0040] 1.1 Experimental method

[0041] 1.1.1 Polypeptide synthesis

[0042] Peptides were synthesized by solid phase synthesis using amide MHBA resin and standard 9-fluorenylmethoxycarbonyl (Fmoc) amino acids. Peptide extension reaction conditions are as follows: standard HBTU / HOBt used as coupling agent, N-dimethylformamide as solvent, 10-fold excess of diisopropylethylamine, 3-fold excess of Fmoc protecting group amino acid or 5-fold excess of free fatty acids. The peptide was cleaved from the resin using a reagent consisting of 87.5% trifluoroacetic acid, 2.5% ethanedithiol, 5% thioanisole, and 5% deionized water, and reacted for 3 hours at room temperature, after which the peptide was exposed to methyl tert-butyl Precipitate in ether-petroleum ether (volume ratio 1:1). The molecular weight of the peptide was confirmed by electrospray ionization mass spectrometry (ESI-MS, Waters, USA). The purity of the peptide was analyzed on a C18 column (250×4.6mm; Shimadzu, Kyoto, ...

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Abstract

The invention discloses short lipopeptid with a specific anti-propionibacterium acne effect and an anti-inflammatory effect, and belongs to the technical field of biology. The short lipopeptid is prepared from C6-C20 fatty acids and an amino acid sequence with a general formula of KWKW. The short lipopeptid can specifically inhibit growth of propionibacterium acnes, and reduce inflammatory response of tissues surrounding hair follicles, and in vitro and in vivo experiments show that the short lipopeptid has high antibacterial and anti-inflammatory activity, and low toxicity; moreover, the short lipopeptid has an action mechanism different from those of traditional antibiotics, resistance to the short lipopeptid is hard to generate, and the short lipopeptid is especially effective for bacterial strains which already generate resistance to the existing antibiotics so that the fundamental purposes of controlling acnes and improving the living quality can be achieved. The short lipopeptidis a compound or complex prepared from lipids and amino acid, can be added into various kinds of wash supplies or make-up, is safe and environmentally friendly, and has wide market prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a short lipopeptide with specific anti-Propionibacterium acnes action and anti-inflammatory action. Background technique [0002] Acne is a common chronic inflammatory skin disease of the pilosebaceous glands in teenagers. It occurs mostly in the head, face, neck and other parts rich in sebaceous glands. In severe cases, skin cancer may also form; (2) It may lead to the formation of acne spots, acne pits, acne scars, etc., affecting young people's career choice, spouse selection, and communication, causing adolescent depression, withdrawn personality, low self-esteem, and affecting the patient's mental health . One of the main pathogenic factors causing acne is Propionibacterium acnes, and moderate to severe inflammatory lesions are currently treated with systemic or topical antibiotics. However, traditional antibiotics are easy to make bacteria resistant, leading to a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/09A61K38/07A61P17/10A61K8/64A61Q19/10A61Q19/00
CPCA61K8/64A61K38/00A61Q19/00A61Q19/10A61P17/10C07K5/0815C07K19/00
Inventor 何坚杨光王静瑜
Owner SOUTHERN MEDICAL UNIVERSITY
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