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Method, model and application of establishing pdia3-specific autoimmune response mouse model

An autoimmune and mouse model technology, applied in the biological field, can solve problems related to the mechanism of influence

Active Publication Date: 2021-07-20
THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides a method, model and application of using PDIA3 protein immunization to establish a mouse-specific autoimmune response model, which solves the key to the study of the mechanism of PDIA3-specific autoimmune responses affecting various tissues and organs of the body, especially the thyroid and brain tissues. means problem

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  • Method, model and application of establishing pdia3-specific autoimmune response mouse model
  • Method, model and application of establishing pdia3-specific autoimmune response mouse model

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Embodiment 1

[0039] A method for establishing a mouse specific autoimmune response model by using PDIA3 protein immunization. In order to investigate the effect of PDIA3-specific autoimmune response on thyroid and brain tissue, we used PDIA3 recombinant protein and Freund's adjuvant to immunize adult CBA / J female mice for related research. CBA / J is an AIT-susceptible mouse strain, and AIT can occur after passive immunization with mouse thyroglobulin (Tg) combined with Freund's adjuvant, so adult CBA / J female mice were selected as experimental subjects in this study.

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Abstract

The invention belongs to the field of biotechnology, and in particular relates to a method for establishing a PDIA3-specific autoimmune response mouse model, a model constructed by the method and an application of the model. The present invention uses PDIA3 to establish a mouse-specific autoimmune response model, and directly explores the possible mechanism of the autoimmune response induced by PDIA3 leading to the impairment of thyroid and brain functions, so that PDIA3 can be used as a clinical diagnosis and treatment of autoimmune thyroiditis (AIT) and AIT. The application of the target in related encephalopathy provides laboratory evidence.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for establishing a protein disulfide bond isomerase A3 (PDIA3) specific autoimmune response mouse model, the model and its application. Background technique [0002] Autoimmune thyroiditis (AIT) is one of the most common endocrine diseases. At present, the reports of AIT-related brain damage are increasing year by year, and its pathogenesis is still unclear. The thyroid autoimmune reaction itself may produce factors that cause damage to the central nervous system, and the cross autoimmune reaction induced by common antigens present in the thyroid and brain tissue Immune response involving the central nervous system is considered the most likely pathogenic mechanism. [0003] Protein disulfide-isomerase A3 (Protein disulfide-isomerase A3, PDIA3) is mainly located in the cavity of the endoplasmic reticulum, can catalyze the formation and isomerization of disulfide...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50G01N33/564G01N33/58G01N33/68
CPCG01N33/5005G01N33/564G01N33/582G01N33/68G01N2800/046G01N2800/70
Inventor 李静单忠艳滕卫平项阳冯颜史晓光李健高艺洋
Owner THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV