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Reagent combination for the determination of carbohydrate antigen 72-4
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A carbohydrate antigen and reagent technology, applied in the field of reagent combinations for the determination of carbohydrate antigen 72-4, can solve problems such as false positive results, achieve the effects of eliminating interference, simplifying components, and improving sensitivity
Active Publication Date: 2022-04-08
SICHUAN MACCURA BIOTECH CO LTD
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Abstract
Description
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Problems solved by technology
Most of the antibodies used in commercial immune detection reagents are animal-derived IgG antibodies. The Fc segment has a high homology with the Fc segment of human IgG and can be recognized and combined by RF, so it is negative in the test substance , In RF-positive test samples, immunoassay reagents may be captured by RF to generate signals, resulting in false positive results
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Embodiment 1
[0055] Embodiment 1 Determination of the preparation of the reagent combination of carbohydrate antigen 72-4
[0056] 1.1 All the components shown in the following Table 1 and the following Table 2 were mixed at the concentrations shown below to prepare the first reagent and the second reagent.
[0057] Table 1: First Reagents
[0058]
[0059] Table 2: Second Reagents
[0060]
[0061]
[0062] 1.2 All the components shown in the following Table 3 and the following Table 4 were mixed at the concentrations shown below to prepare the first reagent and the second reagent.
[0063] Table 3: First Reagents
[0064]
[0065] Table 4: Second Reagents
[0066]
Embodiment 2
[0067] Embodiment 2 Determination of the preparation of the reagent combination of carbohydrate antigen 72-4
[0068] 2.1 All the components shown in the following Table 5 and the following Table 6 were mixed at the concentrations shown below to prepare the first reagent and the second reagent.
[0069] Table 5: First Reagents
[0070]
[0071] Table 6: Second Reagents
[0072]
[0073] 2.2 All the components shown in the following Table 7 and the following Table 8 were mixed at the concentrations shown below to prepare the first reagent and the second reagent.
[0074] Table 7: First Reagents
[0075]
[0076]
[0077] Table 8: Second Reagents
[0078]
[0079] 2.3 All the components shown in the following Table 9 and the following Table 10 were mixed at the concentrations shown below to prepare the first reagent and the second reagent.
[0080] Table 9: First Reagent
[0081]
[0082]
[0083] Table 10: Second Reagents
[0084]
Embodiment 3
[0085] Embodiment 3 Determination of the preparation of the reagent combination of carbohydrate antigen 72-4
[0086] All the components shown in Table 11 below and Table 12 below were mixed at the concentrations shown below to prepare the first reagent and the second reagent.
[0087] Table 11: First Reagent
[0088]
[0089] Table 12: Second Reagents
[0090]
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Abstract
The present invention relates to a reagent combination for determining carbohydrate antigen 72-4. The reagent combination comprises: a first reagent containing a solid phase support, a first anti-saccharide antigen 72-4 antibody, and bovine serum Albumin, Mannitol, PC-300, Glycerol, TX-100, 4-Aminoantipyrine, Tris Buffer, Blocking Agent, and Glycine; Second Reagent Containing Second Anti-Glycine Antigen-like 72‑4 antibody, 2‑morphine ethanesulfonic acid buffer, bovine serum albumin, mannitol, glycerol, TX‑100, and 4‑aminoantipyrine. The reagent combination of the invention is simple in composition, can effectively eliminate the interference of the heterophile antibody in the sample on the detection result, and improve the sensitivity of the detection result.
Description
technical field [0001] The invention relates to the field of immunoassay, in particular to a reagent combination for measuring carbohydrate antigen 72-4. Background technique [0002] Carbohydrate antigen 72-4 (CA72-4) is a tumor-associated glycoprotein obtained by Coleher et al. from liver metastases of breast cancer in 1981. Its molecular weight is greater than 1,000,000, and it belongs to mucin carcinoid embryonic antigen. Histochemical studies have shown that it exists in 50% of breast cancer and 85%-95% of colon, pancreas, stomach, lung and ovarian tumors, not in benign tumors and normal adult tissues. CA72-4 is a broad-spectrum tumor marker. The determination of CA72-4 in serum has clinical application value for ovarian cancer, gastric cancer, rectal cancer, liver cancer, and lung cancer. [0003] CA72-4 laboratory examination methods include radioimmunoassay (RIA), enzyme-linked immunoassay (ELISA), chemiluminescent immunoassay (CLIA), etc. RIA and ELISA appeared ea...
Claims
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