Adipose tissue cryopreservation liquid, cryopreservation method and application of adipose tissue cryopreservation liquid

Abstract

The invention belongs to the field of biotechnology, and particularly relates to adipose tissue cryopreservation liquid, a cryopreservation method and application of the adipose tissue cryopreservation liquid. The adipose tissue cryopreservation liquid is prepared from a DMEM culture medium, sucrose, propylene glycol, proline, tetrahydromethyl pyrimidine, dismutase superoxide, glutathione peroxidase, and tocopherol. No serum is added into the prepared cryopreservation liquid, pathogenic microorganism pollution and rejection reaction are avoided, no DMSO with strong toxicity is added, and thustoxic adipose tissue iss are prevented from being cultured. The cryopreservation liquid has a good cryopreservation effect on the adipose tissues, the adipose tissue iss are high in cell activity andlow in apoptosis rate, a preparation method is simple and easy to operate, and new ideas can be provided for research and development of the field.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a fat tissue cryopreservation solution, a cryopreservation method and an application thereof. Background technique [0002] With the improvement of living standards, people are not only satisfied with the health of the body, but shift more attention to their own image, and the pursuit of beauty is more urgent. Autologous fat is an ideal filling material, which can be used for the treatment of soft tissue defects in almost all parts of the body. Researchers at home and abroad have conducted a lot of research to improve the survival rate and reduce the absorption rate. However, the fat tissue preserved after filling is currently used. In the end, the volume can only be maintained at about 20-30% of that at the time of injection, so in order to achieve a satisfactory filling effect, it is often necessary to perform clinical filling and transplantation again or multiple times. Repeate...

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Problems solved by technology

The method of the invention is simple and easy to operate, the production cost is low, and the prepared adipose tissue has good activity, but the addition of DMSO and human serum albumin in the invention not only increases the toxicity of the adipose tissue, but also easily causes the pollution of pathogenic microorganisms , and cause rejection, which is not conducive to the preservation of adipose tissue

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