Adipose tissue cryopreservation liquid, cryopreservation method and application of adipose tissue cryopreservation liquid
A technology of adipose tissue and cryopreservation method, which is applied in application, preservation of human or animal body, animal husbandry, etc. It can solve the problems of unfavorable adipose tissue preservation, increase of adipose tissue toxicity, pathogenic microorganism contamination, etc., and achieve good cryopreservation effect , eliminate the risk of adipose tissue toxicity, and avoid the effect of pollution
Inactive Publication Date: 2019-02-22
GUANGZHOU RAINHOME PHARM&TECH CO LTD
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Problems solved by technology
The method of the invention is simple and easy to operate, the production cost is low, and the prepared adipose tissue has good activity, but the addition of DMSO and human serum albumin in the invention
Method used
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Embodiment 1
[0028] Prepare a kind of adipose tissue cryopreservation solution, its formula is as follows:
[0029] Sucrose: 5g
[0030] Propylene glycol: 20ml
[0031] Proline: 8g
[0032] Tetrahydromethylpyrimidine: 20ml
[0033] 800IU / mL superoxide dismutase: 1ml
[0034] 750IU IU / mL glutathione peroxidase: 0.5ml
[0035] 3.5mg / mL tocopherol: 2ml
[0036] DMEM medium: 100ml
Embodiment 2
[0038] 100mL DMEM, 2g sucrose, 15mL propylene glycol, 2g proline, 10mL tetrahydromethylpyrimidine, 800IU / mL superoxide dismutase 1mL, 750IU IU / mL glutathione peroxidase 1mL, 3.5mg / mL tocopherol 1mL .
Embodiment 3
[0040] 100mL DMEM, 10g sucrose, 50mL propylene glycol, 10g proline, 35mL tetrahydromethylpyrimidine, 800IU / mL superoxide dismutase 3mL, 750IU / mL glutathione peroxidase 3mL, 3.5mg / mL tocopherol 5mL.
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The invention belongs to the field of biotechnology, and particularly relates to adipose tissue cryopreservation liquid, a cryopreservation method and application of the adipose tissue cryopreservation liquid. The adipose tissue cryopreservation liquid is prepared from a DMEM culture medium, sucrose, propylene glycol, proline, tetrahydromethyl pyrimidine, dismutase superoxide, glutathione peroxidase, and tocopherol. No serum is added into the prepared cryopreservation liquid, pathogenic microorganism pollution and rejection reaction are avoided, no DMSO with strong toxicity is added, and thustoxic adipose tissue iss are prevented from being cultured. The cryopreservation liquid has a good cryopreservation effect on the adipose tissues, the adipose tissue iss are high in cell activity andlow in apoptosis rate, a preparation method is simple and easy to operate, and new ideas can be provided for research and development of the field.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a fat tissue cryopreservation solution, a cryopreservation method and an application thereof. Background technique [0002] With the improvement of living standards, people are not only satisfied with the health of the body, but shift more attention to their own image, and the pursuit of beauty is more urgent. Autologous fat is an ideal filling material, which can be used for the treatment of soft tissue defects in almost all parts of the body. Researchers at home and abroad have conducted a lot of research to improve the survival rate and reduce the absorption rate. However, the fat tissue preserved after filling is currently used. In the end, the volume can only be maintained at about 20-30% of that at the time of injection, so in order to achieve a satisfactory filling effect, it is often necessary to perform clinical filling and transplantation again or multiple times. Repeate...
Claims
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Patent Timeline
Login to view more IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 黄燕飞车七石刘少辉
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
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