A kind of preparation method of tilapia fish scale immunomodulatory peptide

An immune regulation and tilapia technology, applied in the field of immune regulation peptides, can solve problems such as toxic side effects, inapplicability to chronic or preventive uses, and limited use, and achieve the effect of reducing drug costs and significant immune regulation effects

Active Publication Date: 2021-06-18
雷州市龙之润水产品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, toxic side effects and high cost of these drugs limit their use in patients, and most immunomodulatory drugs are not suitable for chronic or preventive use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of tilapia fish scale immunomodulatory peptide, comprising the following steps:

[0025] a) Fish scale pretreatment: take tilapia fish scales and wash them with absolute ethanol and water in sequence, then soak in 0.1mol / L hydrochloric acid for 1 hour, and then drain;

[0026] b) Scale crushing: washing the above-mentioned treated tilapia scales with water until neutral, and then crushing to a scale slurry;

[0027] c) Decalcification of fish scales: add 0.5mol / L citric acid solution to the fish scale slurry so that the liquid level is submerged in the fish scale slurry and 20% higher than the fish scale slurry, fully stirred for 4 hours, and then filtered to separate to obtain the solid content. Wash the solid content once with water and drain it, then add 0.15 mol / L EDTA solution that is 1 times the weight of the solid content to the solid content, stir at 35°C for 1 hour, then filter and wash and drain to prepare decalcified fish scales;

[002...

Embodiment 2

[0032] A preparation method of tilapia fish scale immunomodulatory peptide, comprising the following steps:

[0033] a) Fish scale pretreatment: take tilapia fish scales and wash them with absolute ethanol and water in sequence, then soak in 0.15mol / L hydrochloric acid for 2 hours, and then drain;

[0034] b) Scale crushing: washing the above-mentioned treated tilapia scales with water until neutral, and then crushing to a scale slurry;

[0035] c) Decalcification of fish scales: add 1.5mol / L citric acid solution to the fish scale slurry and make the liquid level submerge the fish scale slurry and be 50% higher than the fish scale slurry, fully stir for 6 hours, then filter and separate to obtain the solid content, Wash the solid content with water for 3 times and drain it, then add 0.20 mol / L EDTA solution 1.5 times the weight of the solid content to the solid content, stir at 40°C for 2 hours, then filter, wash and drain to prepare decalcified fish scales;

[0036] d) Degr...

Embodiment 3

[0040] A preparation method of tilapia fish scale immunomodulatory peptide, comprising the following steps:

[0041] a) Fish scale pretreatment: take tilapia fish scales and wash them with absolute ethanol and water in sequence, then soak in 0.125mol / L hydrochloric acid for 1.5 hours, and then drain;

[0042] b) Scale crushing: washing the above-mentioned treated tilapia scales with water until neutral, and then crushing to a scale slurry;

[0043] c) Decalcification of fish scales: add 1.0mol / L citric acid solution to the fish scale slurry and make the liquid level submerge the fish scale slurry and be 35% higher than the fish scale slurry, fully stir for 5 hours, then filter and separate to obtain the solid content, Wash the solid content twice with water and drain it, then add 1.25 times the weight of the solid content 0.175mol / L EDTA solution to the solid content, stir at 37°C for 1.5 hours, then filter and wash and drain to make decalcified fish scales;

[0044] d) Degr...

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PUM

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Abstract

The invention belongs to the technical field of immunomodulatory peptides. The invention discloses a preparation method of tilapia fish scale immunomodulatory peptide, which comprises the steps of fish scale pretreatment, fish scale crushing, fish scale decalcification, fish scale degreasing, fish scale enzymolysis, post-treatment and the like. The present invention efficiently obtains an immunomodulatory peptide with significant immune regulation effect by deep processing the discarded tilapia fish scales in the processing process, which can be applied to chronic or preventive purposes, and can also be applied to a large number of people, and can reduce the Medication costs.

Description

technical field [0001] The invention relates to the technical field of immunomodulatory peptides, in particular to a preparation method of tilapia scale immunomodulatory peptides. Background technique [0002] The immune system is crucial to our survival and has the function of defending against pathogens, but the immune system is vulnerable to many factors, including stress, unhealthy living habits, pathogens and antigens, etc. Several drugs that have been found to regulate the human immune response include cyclosporine, tacrolimus, glucocorticoids, phytol, aristolochic acid, plumbagin, and levamisole. However, toxic side effects and high cost of these drugs limit their use in patients, and most immunomodulatory drugs are not suitable for chronic or preventive use. Studies have shown that oligopeptides, polypeptides, and some protease hydrolyzate mixed peptides obtained by commercial protease hydrolysis and separation and purification using food-derived proteins as raw mat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 余方苗张壮伟胡旭阳唐云平黄芳芳杨最素丁国芳
Owner 雷州市龙之润水产品有限公司
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