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Application and kit of AHNAK2 gene as colorectal cancer stem cell marker

A stem cell marker and colorectal cancer technology, applied in the medical field, can solve the problems of poor treatment effect, poor precision, high recurrence and metastasis rate, and achieve the effect of high neoantigen affinity and high coverage

Active Publication Date: 2020-05-22
吴式琇
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the overall treatment effect is poor, the recurrence and metastasis rate is high, the side effects of tumor treatment are large, and the precision is poor.

Method used

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  • Application and kit of AHNAK2 gene as colorectal cancer stem cell marker
  • Application and kit of AHNAK2 gene as colorectal cancer stem cell marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The whole exome sequencing data of 1 case of colorectal cancer stem cells combined with the target sequencing data of 20 cases of colorectal cancer stem cells revealed two cancer stem cell-specific somatic mutations in the AHNAK2 gene.

[0023] Specifically, the experimental method of the study mainly includes the following parts: The operation methods not specifically described in the embodiments of the present invention are all operated according to the instrument manual.

[0024] 1. Samples and data sources

[0025] Colorectal cancer samples were all obtained from Hangzhou Cancer Hospital. According to the system stipulated by the ethics review committee, each patient signed an informed consent form before sampling. None of the patients took radiotherapy or chemotherapy, and surgery was the first treatment option.

[0026] 1 case of single-cell sequencing sample and data source:

[0027] a. Sample acquisition: With the informed consent of the patient, firstly, part...

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Abstract

The invention discloses an application of an AHNAK2 gene as a colorectal cancer stem cell marker and a kit, wherein the kit includes a probe for detecting the colorectal cancer stem cell marker and has the sequence represented as the SEQ ID No.1 or 2. Since abnormity of the specific mutation gene, AHNAK2, of colorectal cancer stem cell in Chr14: 105410531 and Chr14: 105410533 is closely related tothe occurrence of the colorectal cancer, the two mutation sites can be applied to the colorectal cancer stem cell marker for assistant diagnosis on the colorectal cancer, so that the application hasvery important significance on the diagnosis of colorectal cancer. Especially, a neoantigen at the S3753P site corresponded to the Chr14: 105410533 A>T mutation has high affinity, and HLA-I cover degree is high, wherein the AHNAK2 is expressed on the surface of the cell.

Description

technical field [0001] The invention belongs to the field of medical technology, and in particular relates to an application of AHNAK2 gene as a marker of colorectal cancer stem cells and a kit. Background technique [0002] Colorectal cancer is one of the most common malignant tumors. There are about 8 million new cases each year in the world, accounting for 10% to 15% of all malignant tumors. Its morbidity and mortality rank third among malignant tumors. [0003] At present, colorectal cancer is mainly treated with comprehensive treatment of surgery and radiation, combined chemotherapy containing 5-Fu, and immunotherapy combined with traditional radiotherapy and chemotherapy. However, the overall treatment effect is poor, the recurrence and metastasis rate is high, the side effects of tumor treatment are large, and the precision is poor. Among them, the occurrence of colorectal cancer is closely related to diet, environment, genetics, etc., and is the result of synergisti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 吴式琇张晓燕
Owner 吴式琇
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