Preparation method of a phycoerythrin ratiometric fluorescent sensor

A phycoerythrin, ratiometric fluorescence technology, applied in chemical instruments and methods, fluorescence/phosphorescence, instruments, etc., to achieve high-efficiency detection, high sensitivity and selectivity, and simple operation.

Active Publication Date: 2019-07-30
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no Fe-based 3 o 4 / B-CDs / SiO 2 - MIPs magnetic molecularly imprinted core / shell polymers to construct ratiometric fluorescent sensors, and domestic and foreign literature and patent reports on the use of ratiometric fluorescence methods to detect phycoerythrin

Method used

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  • Preparation method of a phycoerythrin ratiometric fluorescent sensor
  • Preparation method of a phycoerythrin ratiometric fluorescent sensor
  • Preparation method of a phycoerythrin ratiometric fluorescent sensor

Examples

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Embodiment 1

[0028] This example involves a preparation method of a phycoerythrin ratiometric fluorescent sensor based on magnetic molecularly imprinted core / shell polymers. The schematic diagram of the preparation process and the principle of ratiometric fluorescence detection of phycoerythrin is as follows figure 1 As shown, the specific process steps are as follows:

[0029] Preparation of Aminated Blue Fluorescence Emitting B-CDs: Take 0.3mL of ethylene glycol and 25mL of catechol solution and ultrasonically mix them, transfer them to a 50mL polytetrafluoroethylene liner autoclave, and heat the reaction at 180°C for 12h. Dilute the obtained dark brown mixture with 20 mL of twice-distilled water, centrifuge at 12,000 rpm to remove larger particles, collect the supernatant and filter it with a 0.4 μm microfiltration membrane, and the filtrate is dialyzed with a molecular weight cut-off of 1,000 Da to remove unreacted Experimental raw materials, pour out the solution in the dialysis bag, ...

Embodiment 2

[0033] Example 2: The preparation process of the phycoerythrin ratiometric fluorescence sensor involved in this example and the schematic diagram of the principle of ratiometric fluorescence detection of phycoerythrin, aminated blue fluorescence emission B-CDs and carboxylated Fe 3 o 4 The processing step of magnetic nanoparticle preparation is the same as embodiment 1, wherein B-CDs average size is 3nm, Fe 3 o 4 The average size is 20nm. Other specific process steps are as follows:

[0034] Preparation of magnetic molecularly imprinted core / shell polymer: take 2mL B-CDs aqueous dispersion and add to 18mL containing 0.8mLFe 3 o 4 In the aqueous dispersion of , the mass concentration of B-CDs is 5mg mL -1 , Fe 3 o 4 The mass concentration of magnetic nanoparticles is 15mgmL -1 ; After stirring for 30 minutes, add the template molecule phycoerythrin and 20 μL 3-aminopropyltriethoxysilane, wherein the amount of phycoerythrin is 0.8 μM; continue the reaction for 1 hour, th...

Embodiment 3

[0036] Example 3: The preparation process of the phycoerythrin ratiometric fluorescence sensor involved in this example and the schematic diagram of the principle of ratiometric fluorescence detection of phycoerythrin, aminated blue fluorescence emission B-CDs and carboxylated Fe 3 o 4 The processing step of magnetic nanoparticle preparation is the same as embodiment 1, wherein B-CDs average size is 5nm, Fe 3 o 4 The average size is 25nm. Other specific process steps are as follows:

[0037] Preparation of magnetic molecularly imprinted core / shell polymer: take 2mL B-CDs aqueous dispersion and add to 18mL containing 0.8mLFe 3 o 4 In the aqueous dispersion of , the mass concentration of B-CDs is 8mg mL -1 , Fe 3 o 4 The mass concentration of magnetic nanoparticles is 20mgmL -1 ; After stirring for 30 minutes, add the template molecule phycoerythrin and 20 μL 3-aminopropyltriethoxysilane, wherein the amount of phycoerythrin is 1 μM; continue the reaction for 1 hour, then...

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Abstract

A method for preparing a ratiometric fluorescent sensor for phycoerythrin based on a magnetic molecularly imprinted core-shell polymer is provided. With Fe3O4 magnetic nanoparticles as the core, blue fluorescence-emitting carbon quantum dots (B-CDs) are coupled on the surfaces of Fe3O4 magnetic nanoparticles, and SiO2 shells carrying template molecules (phycoerythrin) are grown on the surfaces of Fe3O4 / B-CDs. Then, the molecularly imprinted polymer SiO2-MIPs are obtained by eluting the template molecules, that is, Fe3O4 / B-CDs / SiO2-MIPs are obtained. Fluorescence emission spectra of the dispersion of Fe3O4 / B-CDs / SiO2-MIPs in the presence of different concentrations of phycoerythrin are measured. By fitting the linear relationship between the ratios Iphycoerythrin / IB-CDs of fluorescence emission peak intensities of phycoerythrin and B-CDs and the molar concentrations of phycoerythrin, the ratiometric fluorescent sensor for phycoerythrin is constructed.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of preparation of nanocomposite materials and molecularly imprinted polymers, in particular to a method for preparing a phycoerythrin ratio fluorescence sensor based on magnetic molecularly imprinted core / shell polymers, and the prepared sensor can be used for the detection of phycoerythrin High sensitivity and high selectivity detection. [0003] Background technique: [0004] At present, the red tide phenomenon caused by the large-scale reproduction of red algae on the ocean surface is becoming more and more serious, and its harm to human health and aquatic resources has aroused widespread social concern. Phycoerythrin is one of the important light-harvesting pigment proteins in seaweed, which together with phycocyanin and allophycocyanin constitute the fluorescent family of phycobiliproteins. These proteins are part of an efficient energy transfer chain that directs the excitation energy of the li...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C09K11/06C08G77/26
CPCC08G77/26C09K11/06C09K2211/14G01N21/6428G01N21/6486C09K11/025C08K3/22C08K2003/2275C08K2201/01G01N2021/6439G01N2600/00
Inventor 金辉桂日军付永鑫卜祥宁
Owner QINGDAO UNIV
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