Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of a phycoerythrin ratiometric fluorescent sensor

A phycoerythrin, ratiometric fluorescence technology, applied in chemical instruments and methods, fluorescence/phosphorescence, instruments, etc., to achieve high-efficiency detection, high sensitivity and selectivity, and simple operation.

Active Publication Date: 2019-07-30
QINGDAO UNIV
View PDF15 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no Fe-based 3 o 4 / B-CDs / SiO 2 - MIPs magnetic molecularly imprinted core / shell polymers to construct ratiometric fluorescent sensors, and domestic and foreign literature and patent reports on the use of ratiometric fluorescence methods to detect phycoerythrin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of a phycoerythrin ratiometric fluorescent sensor
  • Preparation method of a phycoerythrin ratiometric fluorescent sensor
  • Preparation method of a phycoerythrin ratiometric fluorescent sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This example involves a preparation method of a phycoerythrin ratiometric fluorescent sensor based on magnetic molecularly imprinted core / shell polymers. The schematic diagram of the preparation process and the principle of ratiometric fluorescence detection of phycoerythrin is as follows figure 1 As shown, the specific process steps are as follows:

[0029] Preparation of Aminated Blue Fluorescence Emitting B-CDs: Take 0.3mL of ethylene glycol and 25mL of catechol solution and ultrasonically mix them, transfer them to a 50mL polytetrafluoroethylene liner autoclave, and heat the reaction at 180°C for 12h. Dilute the obtained dark brown mixture with 20 mL of twice-distilled water, centrifuge at 12,000 rpm to remove larger particles, collect the supernatant and filter it with a 0.4 μm microfiltration membrane, and the filtrate is dialyzed with a molecular weight cut-off of 1,000 Da to remove unreacted Experimental raw materials, pour out the solution in the dialysis bag, ...

Embodiment 2

[0033] Example 2: The preparation process of the phycoerythrin ratiometric fluorescence sensor involved in this example and the schematic diagram of the principle of ratiometric fluorescence detection of phycoerythrin, aminated blue fluorescence emission B-CDs and carboxylated Fe 3 o 4 The processing step of magnetic nanoparticle preparation is the same as embodiment 1, wherein B-CDs average size is 3nm, Fe 3 o 4 The average size is 20nm. Other specific process steps are as follows:

[0034] Preparation of magnetic molecularly imprinted core / shell polymer: take 2mL B-CDs aqueous dispersion and add to 18mL containing 0.8mLFe 3 o 4 In the aqueous dispersion of , the mass concentration of B-CDs is 5mg mL -1 , Fe 3 o 4 The mass concentration of magnetic nanoparticles is 15mgmL -1 ; After stirring for 30 minutes, add the template molecule phycoerythrin and 20 μL 3-aminopropyltriethoxysilane, wherein the amount of phycoerythrin is 0.8 μM; continue the reaction for 1 hour, th...

Embodiment 3

[0036] Example 3: The preparation process of the phycoerythrin ratiometric fluorescence sensor involved in this example and the schematic diagram of the principle of ratiometric fluorescence detection of phycoerythrin, aminated blue fluorescence emission B-CDs and carboxylated Fe 3 o 4 The processing step of magnetic nanoparticle preparation is the same as embodiment 1, wherein B-CDs average size is 5nm, Fe 3 o 4 The average size is 25nm. Other specific process steps are as follows:

[0037] Preparation of magnetic molecularly imprinted core / shell polymer: take 2mL B-CDs aqueous dispersion and add to 18mL containing 0.8mLFe 3 o 4 In the aqueous dispersion of , the mass concentration of B-CDs is 8mg mL -1 , Fe 3 o 4 The mass concentration of magnetic nanoparticles is 20mgmL -1 ; After stirring for 30 minutes, add the template molecule phycoerythrin and 20 μL 3-aminopropyltriethoxysilane, wherein the amount of phycoerythrin is 1 μM; continue the reaction for 1 hour, then...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
sizeaaaaaaaaaa
Login to View More

Abstract

The invention specifically relates to a method for preparing a phycoerythrin ratiometric fluorescence sensor based on magnetic molecularly imprinted core / shell polymers. in Fe 3 O 4 The surface of magnetic nanoparticles is coupled with blue fluorescent carbon quantum dots B-CDs, and SiO containing the template molecule phycoerythrin is grown on the surface of the conjugate. 2 The shell layer is eluted and the template is eluted to prepare a magnetic molecularly imprinted core / shell polymer. The fluorescence emission spectra of the polymer dispersion at different phycoerythrin concentrations were measured, and the fluorescence peak intensity ratio I of phycoerythrin and B‑CDs was fitted. 藻红蛋白 / I B‑CDs Linear relationship with the molar concentration of phycoerythrin to construct a ratiometric fluorescence sensor for detecting phycoerythrin. Compared with the existing technology, the method of the present invention is simple to operate, low in cost, easy to obtain raw materials, strong anti-interference ability of the ratio signal, good accuracy, high sensitivity and selectivity. The ratio fluorescence sensor of the invention can be used for the detection of phycoerythrin. Efficient detection.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of preparation of nanocomposite materials and molecularly imprinted polymers, in particular to a method for preparing a phycoerythrin ratio fluorescence sensor based on magnetic molecularly imprinted core / shell polymers, and the prepared sensor can be used for the detection of phycoerythrin High sensitivity and high selectivity detection. [0003] Background technique: [0004] At present, the red tide phenomenon caused by the large-scale reproduction of red algae on the ocean surface is becoming more and more serious, and its harm to human health and aquatic resources has aroused widespread social concern. Phycoerythrin is one of the important light-harvesting pigment proteins in seaweed, which together with phycocyanin and allophycocyanin constitute the fluorescent family of phycobiliproteins. These proteins are part of an efficient energy transfer chain that directs the excitation energy of the li...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C09K11/06C08G77/26
CPCG01N21/6428G01N21/6486C09K11/06C08G77/26C09K2211/14C09K11/025C08K3/22C08K2003/2275C08K2201/01G01N2021/6439G01N2600/00
Inventor 金辉桂日军付永鑫卜祥宁
Owner QINGDAO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com