Preparation method of vanillin ratiometric electrochemical aptasensor based on nanocomposite modified electrode

A nanocomposite and aptamer sensor technology, which is applied in the cross field of nanocomposite materials and electrochemical sensors, to achieve the effects of high electrochemical activity, high sensitivity and simple operation

Active Publication Date: 2019-07-12
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no ratiometric electrochemical aptamer sensing method for the detection of vanillin, and a ratiometric electrochemical aptasensor based on aptamer / gold nanoparticles / ferrocene / ketjen black / ZIF-8 nanocomposite modified electrodes Reports on domestic and foreign literature and patents

Method used

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  • Preparation method of vanillin ratiometric electrochemical aptasensor based on nanocomposite modified electrode
  • Preparation method of vanillin ratiometric electrochemical aptasensor based on nanocomposite modified electrode
  • Preparation method of vanillin ratiometric electrochemical aptasensor based on nanocomposite modified electrode

Examples

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Effect test

Embodiment 1

[0027] For the preparation of a vanillin ratio electrochemical aptamer sensor based on aptamer / gold nanoparticles / ferrocene / ketjen black / ZIF-8 nanocomposite modified electrode and vanillin ratio electrochemical detection, The schematic diagram of its preparation process and principle is shown in figure 1 As shown, the specific process steps are:

[0028] Dissolve 2-methylimidazole (Hmim) in ethanol, add ferrocene and Ketjen black, stir fully until the mixture is homogeneous, add zinc nitrate, stir evenly and transfer it to a high-pressure reactor, in which 2-methylimidazole The mass concentration ratio of ferrocene / ketjen black / zinc nitrate is 130:200:1:60. The reaction was continued at 90° C. for 6 h, and the reaction product was centrifuged, washed with ethanol, and dried in vacuum to obtain a ZIF-8 nanocomposite loaded with ferrocene and Ketjen black.

[0029] Add 2 microliters of cross-linking agent Nafion to the surface of the bare glassy carbon electrode after polishin...

Embodiment 2

[0032] In this example, the preparation of the vanillin ratio electrochemical aptasensor based on the nanocomposite modified electrode and the vanillin ratio electrochemical detection, the preparation process and schematic diagram are the same as in Example 1, and the specific process steps are:

[0033] Dissolve 2-methylimidazole (Hmim) in ethanol, add ferrocene and Ketjen black, stir fully until the mixture is homogeneous, add zinc nitrate, stir evenly and transfer it to a high-pressure reactor, in which 2-methylimidazole The mass concentration ratio of ferrocene / ketjen black / zinc nitrate is 150:300:2:80. The reaction was continued at 120° C. for 12 hours, and the reaction product was centrifuged, washed with ethanol, and dried in vacuum to obtain a ZIF-8 nanocomposite loaded with ferrocene and Ketjen Black.

[0034] Add 5 microliters of cross-linking agent Nafion to the surface of the bare glassy carbon electrode after polishing and polishing, and then drop-coat the ZIF-8 n...

Embodiment 3

[0037] In this example, the preparation of the vanillin ratio electrochemical aptasensor based on the nanocomposite modified electrode and the vanillin ratio electrochemical detection, the preparation process and schematic diagram are the same as in Example 1, and the specific process steps are:

[0038] Dissolve 2-methylimidazole (Hmim) in ethanol, add ferrocene and Ketjen black, stir fully until the mixture is homogeneous, add zinc nitrate, stir evenly and transfer it to a high-pressure reactor, in which 2-methylimidazole The mass concentration ratio of ferrocene / ketjen black / zinc nitrate is 200:400:3:100. The reaction was continued at 150° C. for 8 hours, and the reaction product was centrifuged, washed with ethanol, and dried in vacuum to obtain a ZIF-8 nanocomposite loaded with ferrocene and Ketjen Black.

[0039] Add 8 microliters of cross-linking agent Nafion dropwise on the surface of the bare glassy carbon electrode after polishing and polishing, and then drop-coat th...

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Abstract

The invention specifically relates to a method for preparing a vanillin ratio electrochemical aptamer sensor based on aptamer / gold nanoparticles / ferrocene / ketjen black / ZIF-8 nanocomposite modified electrode. Drop-coat ferrocene- and Ketjen black-doped ZIF‑8 nanocomposites on the surface of the bare glassy carbon electrode, immerse the modified electrode in chloroauric acid solution, and use cyclic voltammetry scanning to electrodeposit gold nanoparticles to prepare gold nanoparticles Particle-deposited ZIF-8 nanocomposites. The vanillin aptamer was linked to the gold nanoparticles through Au‑S bonds to construct an aptamer-coupled nanocomposite sensing platform. The electrochemical curves at different concentrations of vanillin were measured, and the linear relationship between the ratio of the current peak intensity of vanillin and ferrocene and the concentration of vanillin was fitted to construct a ratiometric electrochemical aptasensor for vanillin. Compared with the prior art, the method of the invention has simple operation, low cost, good ratio signal stability and high sensitivity, and can be used as a novel ratio electrochemical aptasensor for efficient detection of vanillin.

Description

[0001] Technical field: [0002] The invention belongs to the cross technical field of nanocomposite materials and electrochemical sensors, in particular to a vanillin ratio electrochemical aptamer based on aptamer / gold nanoparticles / ferrocene / ketjen black / ZIF-8 nanocomposite modified electrode The preparation method of the sensor, the prepared sensor can be used for efficient detection of vanillin. [0003] Background technique: [0004] Metal-organic frameworks (MOFs) have the advantages of large specific surface area, high porosity, ordered crystal structure, and good mechanical stability. Due to the generally poor conductivity of MOFs, the research and application of MOFs in electrochemical sensing are rarely reported. As a zeolite-like MOFs, ZIF-8 has excellent thermal and chemical stability, and the composite prepared by mixing it with electroactive substances can significantly improve the conductivity and electrochemical sensing performance of ZIF-8. Ketjen black is a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/327G01N27/3278G01N33/5308G01N33/5438
Inventor 桂日军孙玉娇金辉姜晓文
Owner QINGDAO UNIV
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