Preparation method of phycoerythrin ratio fluorescence sensor based on magnetic molecular imprinted nucleus/shell polymer

A magnetic molecular imprinting, phycoerythrin technology, applied in chemical instruments and methods, fluorescence/phosphorescence, instruments, etc., to achieve the effects of easy availability of raw materials, high sensitivity and selectivity, and simple operation

Active Publication Date: 2019-03-01
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no Fe-based 3 o 4 / B-CDs / SiO 2 - MIPs magnetic molecularly imprinted core / shell polymers to construct ratiometric fluorescent sensors, and domestic and foreign literature and patent reports on the use of ratiometric fluorescence methods to detect phycoerythrin

Method used

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  • Preparation method of phycoerythrin ratio fluorescence sensor based on magnetic molecular imprinted nucleus/shell polymer
  • Preparation method of phycoerythrin ratio fluorescence sensor based on magnetic molecular imprinted nucleus/shell polymer
  • Preparation method of phycoerythrin ratio fluorescence sensor based on magnetic molecular imprinted nucleus/shell polymer

Examples

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Embodiment 1

[0023] This example involves a preparation method of a phycoerythrin ratiometric fluorescent sensor based on magnetic molecularly imprinted core / shell polymers. The schematic diagram of the preparation process and the principle of ratiometric fluorescence detection of phycoerythrin is as follows figure 1 As shown, the specific process steps are as follows:

[0024] Preparation of Aminated Blue Fluorescence Emitting B-CDs: Take 0.3mL of ethylene glycol and 25mL of catechol solution and ultrasonically mix them, transfer them to a 50mL polytetrafluoroethylene liner autoclave, and heat the reaction at 180°C for 12h. Dilute the obtained dark brown mixture with 20 mL of twice-distilled water, centrifuge at 12,000 rpm to remove larger particles, collect the supernatant and filter it with a 0.4 μm microfiltration membrane, and the filtrate is dialyzed with a molecular weight cut-off of 1,000 Da to remove unreacted Experimental raw materials, pour out the solution in the dialysis bag, ...

Embodiment 2

[0028] Example 2: The preparation process of the phycoerythrin ratiometric fluorescence sensor involved in this example and the schematic diagram of the principle of ratiometric fluorescence detection of phycoerythrin, aminated blue fluorescence emission B-CDs and carboxylated Fe 3 o 4 The processing step of magnetic nanoparticle preparation is the same as embodiment 1, wherein B-CDs average size is 3nm, Fe 3 o 4 The average size is 20nm. Other specific process steps are as follows:

[0029] Preparation of magnetic molecularly imprinted core / shell polymer: take 2mL B-CDs aqueous dispersion and add to 18mL containing 0.8mLFe 3 o 4 In the aqueous dispersion of , the mass concentration of B-CDs is 5 mg mL -1 , Fe 3 o 4 The mass concentration of magnetic nanoparticles is 15mgmL -1 ; After stirring for 30 minutes, add the template molecule phycoerythrin and 20 μL 3-aminopropyltriethoxysilane, wherein the amount of phycoerythrin is 0.8 μM; continue the reaction for 1 hour, t...

Embodiment 3

[0031] Example 3: The preparation process of the phycoerythrin ratiometric fluorescence sensor involved in this example and the schematic diagram of the principle of ratiometric fluorescence detection of phycoerythrin, aminated blue fluorescence emission B-CDs and carboxylated Fe 3 o 4 The processing step of magnetic nanoparticle preparation is the same as embodiment 1, wherein B-CDs average size is 5nm, Fe 3 o 4 The average size is 25nm. Other specific process steps are as follows:

[0032] Preparation of magnetic molecularly imprinted core / shell polymer: take 2mL B-CDs aqueous dispersion and add to 18mL containing 0.8mLFe 3 o 4 In the aqueous dispersion of , the mass concentration of B-CDs is 8 mg mL -1 , Fe 3 o 4 The mass concentration of magnetic nanoparticles is 20mgmL -1 ; After stirring for 30 minutes, add template molecule phycoerythrin and 20 μL 3-aminopropyltriethoxysilane, wherein the amount of phycoerythrin is 1 μM; continue to react for 1 hour, then add 40...

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Abstract

The invention relates to a preparation method of a phycoerythrin ratio fluorescence sensor based on a magnetic molecular imprinted nucleus/shell polymer. A Fe3O4 magnetic nano-particleis used as the center, the surface of the Fe3O4 magnetic nano-particleis coupled with blue fluorescence emitting carbon quantum dots B-CDs, a SiO2 shell layer loaded with template molecule phycoerythrin generates onthe surfaces of the Fe3O4/B-CDs, and template molecule elution is conducted to prepare magnetic molecular imprinted nucleus/shell polymer Fe3O4/B-CDs/SiO2-MIPs. The polymer dispersion liquid fluorescence emission spectra under different phycoerythrin concentration are measured, the linear relation between the fluorescence emission peak intensity ratio I<phycoerythrin>/I<B-CDs> of the phycoerythrinto the B-CDs and the phycoerythrin molar concentration is fitted, and a phycoerythrin ratio fluorescence sensor is constructed. Compared with the prior art, the method is simple to operate and low incost, raw materials are easy to obtain, ratio signal interference resisting capacity is high, accuracy is high, flexibility and selectivity are high, and a novel ratio fluorescence sensor can be developed and used for efficient phycoerythrin detection.

Description

Technical field: [0001] The invention belongs to the technical field of preparation of nanocomposite materials and molecularly imprinted polymers, in particular to a method for preparing a phycoerythrin ratio fluorescence sensor based on magnetic molecularly imprinted core / shell polymers, and the prepared sensor can be used for the detection of phycoerythrin High sensitivity and high selectivity detection. Background technique: [0002] At present, the red tide phenomenon caused by the large-scale reproduction of red algae on the ocean surface is becoming more and more serious, and its harm to human health and aquatic resources has aroused widespread social concern. Phycoerythrin is one of the important light-harvesting pigment proteins in seaweed, which together with phycocyanin and allophycocyanin constitute the fluorescent family of phycobiliproteins. These proteins are part of an efficient energy transfer chain that directs the excitation energy of the light-harvesting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/06C08G77/26
CPCC08G77/26C09K11/06C09K2211/14G01N21/6428G01N21/6486C09K11/025C08K3/22C08K2003/2275C08K2201/01G01N2021/6439G01N2600/00
Inventor 金辉桂日军付永鑫卜祥宁
Owner QINGDAO UNIV
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