Construction method and detection method of uplc characteristic map of plantain medicinal material

Abstract

The invention relates to a construction method and detection method of UPLC characteristic map of plantain medicinal material. The steps of the characteristic map construction method are as follows: prepare the reference substance reference solution with psyllium and verbascoside as reference substances respectively; prepare the reference substance reference solution with the plantain control medicinal material; The sample is extracted with an extraction solvent, filtered, and the resulting filtrate is used as the test solution and the standard decoction test solution; respectively draw the reference solution, the test solution, and the standard decoction test solution into the ultra-high performance liquid phase Chromatograph, determination; compare the test spectrum of the plantain medicinal material with the standard decoction sample test spectrum, calibrate the characteristic peaks of 7 water-soluble components, and obtain the UPLC characteristic spectrum of the plantain medicinal material. The characteristic spectrum can be used for qualitative and quantitative analysis of the quality of the plantain medicinal material, can ensure the quality of the traditional plantain decoction prepared by using the medicinal material, and is also suitable for detecting other preparations containing the plantain grass.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a construction method and a detection method of a UPLC characteristic map of plantain medicinal material. Background technique [0002] Plantain is the dry whole grass of Plantago asiatica L. or Plantago depressaWilld. Plantain was first seen in "The Book of Songs·Zhou Nan·芣苢", and "Shen Nong's Materia Medica" was listed as the top grade. "Compendium of Materia Medica" states: "People in Youzhou call it Buglossis, and toads like to hide under it, so Jiangdong is called toad clothing; the smell is sweet, cold, and non-toxic. It is mainly used to relieve pain, improve water passages and urinate, and eliminate dampness. Take it for a long time to lighten the body and endure old age. Men are injured, women are drenched and don’t want to eat. It nourishes the lungs, strengthens yin and nourishes essence, makes people have children, improves eyesight and cures red pain. Removes wind ...

AI Technical Summary

Problems solved by technology

[0004] The 2015 edition of "Chinese Pharmacopoeia" uses psyllium as the quality evaluation index of psyllium, and only controls the content of psyllium, which cannot fully reflect its quality, and the establishment of the characteristic map of cypresses reported in the current literature research, All of them are only for the raw materials of medicinal materials, and most of the index components are fat-soluble components as the research objects. They are mainly used for qualitative identification of authenticity, origin, and quality differences of Chinese medicinal materials. It is difficult to fully reflect the quality characteristics of plantain grass, and it cannot reflect the traditional Chinese medicine soup. The material basis characteristics of the agent

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