Selenium sodium alginate and selenium chitosan coated probiotic double-layer microcapsule as well as preparation method and application thereof

A technology for selenizing chitosan and sodium alginate, which is applied in the field of medicine and biology, and achieves the effects of good biosafety and biocompatibility, large embedding capacity, and improved survival rate.

Active Publication Date: 2019-03-12
广东南芯医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the improvement of intestinal microecology is a complex system engineering. In addition to the colonization of probiotics, it is often necessary to add functional molecules, enhance immunity and other auxiliary means to achieve a synergistic effect
The currently disclosed probiotic coatings and their preparations do not have this function

Method used

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  • Selenium sodium alginate and selenium chitosan coated probiotic double-layer microcapsule as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Dissolve 0.3g of sodium selenite in a small amount of water, slowly add it to an aqueous solution of sodium alginate with a weight-average molecular weight of 20,000, filter the filtrate, add a 70% ethanol solution for ethanol analysis overnight, filter it with Rinse repeatedly with absolute ethanol, dry at low temperature, and pulverize to obtain selenized sodium alginate.

[0033] 2. get 0.3g sodium selenite and be dissolved in a small amount of water, slowly join in the chitosan aqueous solution that weight-average molecular weight is 10,000, filter and get the filtrate, add concentration and be 70% ethanol solution alcohol analysis overnight, suction filtration, use Rinse with absolute ethanol repeatedly, dry at low temperature, and pulverize to obtain selenized chitosan.

[0034] 3. Preparation of 1% selenized sodium alginate and 0.5% selenized chitosan solution, sterilized at 115°C for 30 minutes, filled with nitrogen and deoxygenated, to obtain sterile seleniz...

Embodiment 2

[0040] 1. Dissolve 0.6g of sodium selenite in a small amount of water, and slowly add it to sodium alginate with a weight average molecular weight of 400,000. After reacting for 2 hours, filter the filtrate, add 100% ethanol solution for ethanol analysis overnight, and filter with suction , washed repeatedly with absolute ethanol, dried at low temperature, and crushed to obtain sodium selenide alginate.

[0041] 2. Dissolve 0.6g of sodium selenite in a small amount of water, slowly add it to a chitosan solution with a weight average molecular weight of 200,000, react for 2 hours, filter the filtrate, add a 100% ethanol solution for alcohol analysis overnight, and filter with suction , rinsed repeatedly with absolute ethanol, dried at low temperature, and crushed to obtain chitosan selenide.

[0042] 3. Preparation of 5% selenized sodium alginate and 2% selenized chitosan solution, sterilized at 115°C for 30 minutes, filled with nitrogen and deoxygenated to obtain sterile selen...

Embodiment 3

[0048] 1. Dissolve 0.4g of sodium selenite in a small amount of water, slowly add it to sodium alginate with a weight-average molecular weight of 100,000, react for 1.5 hours and then filter the filtrate, add a 95% ethanol solution for alcohol analysis overnight, and filter with suction , washed repeatedly with absolute ethanol, dried at low temperature, and crushed to obtain sodium selenide alginate.

[0049]2. Dissolve 0.4g of sodium selenite in a small amount of water, slowly add it to a chitosan solution with a weight-average molecular weight of 100,000, react for 1.5 hours and filter the filtrate, add a 95% ethanol solution for alcohol analysis overnight, and filter with suction , rinsed repeatedly with absolute ethanol, dried at low temperature, and crushed to obtain chitosan selenide.

[0050] 3. Preparation of 3% selenized sodium alginate and 1.5% selenized chitosan solution, sterilized at 115°C for 30 minutes, filled with nitrogen and deoxygenated, to obtain sterile s...

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Abstract

The invention discloses a selenium sodium alginate and selenium chitosan coated probiotic double-layer microcapsule as well as a preparation method and application thereof. The preparation method comprises the following steps of treating sodium alginate and chitosan with sodium selenite to prepare selenium sodium alginate and selenium chitosan; embedding probiotics with the selenium sodium alginate and the selenium chitosan; and using Ca<2+> crosslinking to acquire the probiotic double-layer microcapsule. The selenium sodium alginate and selenium chitosan coated probiotic double-layer microcapsule provided by the invention can exert the effect of probiotic colonization to improve intestinal microecology and can further replenish selenium elements essential for a human body to enhance the immunity of an organism and further strengthen the gain effect of the probiotics to the human body.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to a probiotic bilayer microcapsule coated with selenized sodium alginate and selenized chitosan, a preparation method and an application thereof. Background technique [0002] Probiotics change the composition of the flora in a certain part of the host through colonization, thereby producing a single or a mixture of microorganisms with a clear composition that is beneficial to the health of the host. As the organ with the highest abundance of microbial flora in the human body, proper supplementation of probiotics can improve intestinal function and have positive significance for maintaining human health. However, there are digestive juices such as gastric acid and bile salts in the human digestive tract, which have a strong antibacterial and bactericidal effect, which poses a huge obstacle to the colonization of oral probiotics. [0003] Sodium alginate is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/744A61K35/747A61K35/745A61K9/62A61K47/36A61K47/02A61P1/00A61P37/04A61K33/04
CPCA61P1/00A61P37/04A61K9/501A61K9/5036A61K9/5073A61K9/5089A61K33/04A61K35/744A61K35/745A61K35/747A61K2300/00
Inventor 吴剑徐阳刁嘉茵陈宏波
Owner 广东南芯医疗科技有限公司
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