A method for improving the induction effect of agapanthus body embryos

A technique for embryo seedlings and seedlings, which is applied in the field of improving the induction effect of Agacia japonica body embryo seedlings, can solve the problems of embryo abortion, the number of induced mature embryos is small, the number of somatic embryo seedlings is low and the like, and the number of mature seedlings can be increased and reduced. The occurrence of deformed seedlings and the effect of hormone regulation optimization

Active Publication Date: 2021-12-10
XINYANG AGRI & FORESTRY UNIV
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Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the number of mature embryos induced by embryogenic cells of Agapanthus in the process of frequent subculture is small, the number of embryos aborted and the number of somatic embryos induced is low, and a method for improving the induction of somatic embryos of Agapanthus is provided. effect method

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  • A method for improving the induction effect of agapanthus body embryos
  • A method for improving the induction effect of agapanthus body embryos
  • A method for improving the induction effect of agapanthus body embryos

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Embodiment 1

[0032] A method for improving the induction effect of agapanthus body embryos, comprising the steps of:

[0033] (1) Material collection of explants: during the flowering period in May to June, uncracked small flower buds of Agapanthus 4 to 5 years old were taken, and disinfected on an aseptic operating table (firstly treated with 75% (v / v) ethanol for 1 min) , followed by ddH 2 O rinsed 4 times, then disinfected with 2% sodium hypochlorite for 5min), and then treated with ddH 2 0 rinse 4 times, blot the moisture on the surface of the small flower buds with sterile filter paper, then cut the small pedicel explants, and cut the small pedicel explants into small sections of 0.7~1.0cm;

[0034] (2) Callus induction: Take 0.7-1.0 cm small pedicel explants and inoculate them in the callus induction medium in a flat state, and culture them in the dark at 25°C for 15 days, white translucent callus can be seen ( figure 1 It is an anatomical microscope observation figure after the s...

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Abstract

The invention relates to a method for improving the effect of inducing embryonic seedlings of Agapanthus lily, which comprises the extraction of explants, the induction of callus, the subculture of callus, the induction of embryogenic callus, and the maturation of embryogenic cells Embryo induction and somatic embryo seedling induction and other steps. According to the hormone synthesis and metabolism and signal transduction characteristics of Agapanthus agapanthus embryogenic initiation and maintenance, the present invention uses small pedicels as explants to induce callus tissue, and obtains embryogenic callus tissue through subculture and induction of callus tissue. During the embryo induction period, the ratio of sugar and hormones is changed, and the polarity of cells is adjusted to induce a large number of somatic embryos with synchronous development. Synthesize and signal to obtain somatic embryo seedlings with complete shape, good growth and a large number. The invention solves the problems that the number of induced mature embryos, embryo abortion and somatic embryo seedlings produced in the process of frequent subculture of the embryogenic cells of Agapanthus are small.

Description

technical field [0001] The invention belongs to the technical field of rapid multiplication of improved plant varieties, and in particular relates to a method for improving the induction effect of agapanthus body embryo seedlings. Background technique [0002] Agapanthus praecox ssp.orientalis, also known as "Blue Lily" and "African Lily", is native to South Africa and the Kingdom of Lesotho in southern Africa. It is a perennial herbaceous monocot flower with strong ornamental value. For nearly half a century, Agapanthus has stood out in the development of the international flower industry and has become a popular fresh-cut flower, potted plant and ground cover flower all over the world, and it has shown excellent ornamental value. Agapanthus is heat-resistant, drought-resistant, and has strong cold resistance. It has huge development space in the fields of road greening and soil restoration. At present, the supply of seedlings in the market is in short supply. [0003] Aga...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 龚守富岳建华董艳张琰李蒙夏维丽施芹川王苗
Owner XINYANG AGRI & FORESTRY UNIV
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