Paenibacillus polymyxa fermentation product and its preparation method and application

A technology for polymyxa spore and fermented product, which is applied to the field of polymyxa spore fermented product and its preparation, can solve problems such as limited application, poor content penetration, etc., and achieves the promotion of plant growth, good control effect, increased Effects of Rice Yield

Active Publication Date: 2020-08-18
ZHEJIANG XINNONG CHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Conventional liposomes, as a good carrier, have many unique advantages, but at the same time, there are certain deficiencies. Over time, there are stability problems such as flocculation, sedimentation, and content penetration caused by particle size increase. The problem of poor performance limits its application in the pharmaceutical and pesticide industries

Method used

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  • Paenibacillus polymyxa fermentation product and its preparation method and application
  • Paenibacillus polymyxa fermentation product and its preparation method and application
  • Paenibacillus polymyxa fermentation product and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Indoor bioassay of embodiment 1 Paenibacillus polymyxa

[0048] 1. Isolation of endophytes from rice

[0049] Pick healthy rice plants from the bad seedlings of rice and sheath blight diseased fields in Wenling and Jinhua, Zhejiang, take the leaves of the upper and lower middle parts of the plants, cut them into small pieces of 5 mm × 5 mm, disinfect them with 75% ethanol for about 1 min, and then Rinse 3 times with sterilized water to remove residual disinfectant, and finally put it on sterile absorbent paper to absorb excess water, then put it into a sterile mortar, add 6mL of BPS buffer to grind, take The supernatant was diluted 10, 50, and 100 times, and 200 μL of each dilution was applied to the LB plate. At the same time, verify that the last rinse water was applied to the LB plate to check whether the surface of the tissue was thoroughly disinfected. Incubate in the dark at 30°C for 1-2 days, and record Colony morphology, size and color.

[0050] The biological...

Embodiment 2

[0057] The fermentation process of embodiment 2 Paenibacillus polymyxa (ZJU-N)

[0058] See Table 1 for the medium used in the fermentation of this embodiment.

[0059] Table 1 The medium used for the fermentation of this embodiment is summarized

[0060]

[0061] Equipment: Paenibacillus polymyxa (ZJU-N) liquid complete fermentation equipment was purchased from Shanghai Chuyi Biotechnology Co., Ltd.

[0062] Fermentation process:

[0063] (1) Strain activation: pick a ring of Paenibacillus polymyxa ZJU-N and streak it on a solid plate for activation, and place the inoculated plate in a 30°C incubator for 72 hours;

[0064] (2) Seed culture: Pick an independent colony of Paenibacillus polymyxa activated in step (1) and inoculate it in a 250ml Erlenmeyer flask with 100ml seed culture solution, and cultivate it for 24h at a temperature of 30°C and a rotation speed of 160rpm ;

[0065] (3) Fermentation culture: insert the cultivated seed solution into a 15L fermenter conta...

Embodiment 3

[0067] The optimization of embodiment 3 fermentation process

[0068] The optimization of the fermentation medium in this embodiment: in the basal medium (tryptone: 5g / L; beef extract: 3g / L; yeast extract: 1g / L; NaCl: 3g / L; NaCl: 3g / L; 2 HPO 4 .12H 2 O: 1.5g; Ca(NO 3 ) 2 .4H 2 (2: 0.5g) on ​​the basis of investigating several common carbon sources (the consumption of carbon source is the same as embodiment 2) on the influence of Paenibacillus polymyxa viable count, spore production amount and bacterial film secretion. See Table 2. All the other steps are identical to Example 2.

[0069] Table 2: Effects of different carbon sources on the number of viable bacteria, spore production and biofilm secretion of Paenibacillus polymyxa (ZJU-N)

[0070]

[0071] Note: The amount of biofilm secretion is more "+++", more "++", less "+", no secretion "-"

[0072] As can be seen from the above table 2, potato and sucrose are comparatively suitable carbon sources. Simultaneously,...

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Abstract

The invention discloses fermented paenibacillus poly myxa and a preparation method and application thereof. The preparation method of the fermented panebacillus polymyxa comprises the following steps:carrying out activation, seed culture and fermentation culture on a strain in sequence, and centrifuging and concentrating, thus obtaining the fermented panebacillus polymyxa; the fermented panebacillus polymyxa is a mixture of pellicle and panebacillus polymyxa sporophore; the strain is paenibacillus poly myxa ZJU-N. The fermented panebacillus polymyxa, the preparation method and the applicationdisclosed by the invention have the beneficial effects that by selection of the specific paenibacillus poly myxa and change of the component types and use amounts of a seed culture medium and a fermentation culture medium for fermentation, a fermenting product containing the rich pellicle and paeniebacillus poly myxa sporophore can be obtained and is used for preparing sporophore microcapsules and sporophore microcapsule suspending agents to prevent and control banded sclerotial blight, green smut and bakanae disease of rice.

Description

technical field [0001] The invention relates to the technical field of biological control of crop diseases, in particular to a fermented product of Paenibacillus polymyxa and its preparation method and application. Background technique [0002] Biological pesticides refer to: "A type of pesticide preparation that uses living organisms or their metabolites to control pests, germs, weeds and other harmful organisms, or biological products that have the function of pesticides through biotechnology or bionic synthesis." In the management of pesticide registration, the concept of biological pesticides is usually narrowly defined, mainly referring to insect natural enemies, botanical pesticides, microbial pesticides and genetically modified organisms. [0003] Recently, although domestic chemical pesticides have played an important role, the problem of environmental pollution has become more and more prominent. The green development of biological pesticides is the need for food se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N25/04A01N25/28A01N63/25A01P3/00
CPCA01N25/04A01N25/28A01N63/10
Inventor 林婷陈梦丽乔丽雅朱瞬瞬胡斌谢烽朱国念
Owner ZHEJIANG XINNONG CHEM CO LTD
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