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Application of human amniotic epithelial cell secretory factor in preparation of medicine for ovarian function restoration

A technology of epithelial cells and secreting factors, which is applied in the field of biomedicine to achieve the effects of promoting angiogenesis, significant ovarian repair, and preserving cell activity

Inactive Publication Date: 2019-03-29
THE INT PEACE MATERNITY & CHILD HEALTH HOSPITAL OF CHINA WELFARE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no relevant report on the use of human amniotic epithelial cell-secreted factors to prepare drugs for restoring ovarian function

Method used

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  • Application of human amniotic epithelial cell secretory factor in preparation of medicine for ovarian function restoration
  • Application of human amniotic epithelial cell secretory factor in preparation of medicine for ovarian function restoration
  • Application of human amniotic epithelial cell secretory factor in preparation of medicine for ovarian function restoration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Preparation of Human Amniotic Epithelial Cell Secreted Factors (1)

[0057] 1. With the approval of the Ethics Committee of our hospital and the informed consent of the research subjects, fresh amniotic tissue from full-term healthy puerperas was obtained under sterile conditions.

[0058] 2. Digest amnion tissue with 0.25% trypsin for 1.5min to separate human amniotic epithelial cells, resuspend in DMEM / F12 (1:1) medium containing 10% fetal bovine serum, and inoculate in 100mm cell culture dish placed in a 5% CO 2 cultured in a 37°C cell culture incubator.

[0059] 3. When the cell confluence reaches 70%-80%, use 0.25% trypsin to digest the adherent cells for 2.5 minutes and resuspend them in DMEM / F12 (1:1) medium containing 10% fetal bovine serum to readjust the cells Density is 1×10 5 After inoculation in 100mm cell culture dish, placed in 5% CO 2 cultured in a 37°C cell culture incubator. At the same time, some human amniotic epithelial cells were col...

Embodiment 2

[0062] Example 2 Preparation of Human Amniotic Epithelial Cell Secreted Factors (2)

[0063] 1. With the approval of the Ethics Committee of our hospital and the informed consent of the research subjects, fresh amniotic tissue from full-term healthy puerperas was obtained under sterile conditions.

[0064] 2. Digest amnion tissue with 0.25% trypsin for 2 minutes to separate human amniotic epithelial cells, resuspend in DMEM / F12 (1:1) medium containing 10% fetal bovine serum, inoculate in 100mm cell culture dish and place at 5% CO 2 cultured in a 37°C cell culture incubator.

[0065] 3. When the cell confluence reaches 70%-80%, use 0.25% trypsin to digest the adherent cells for 3 minutes and resuspend in DMEM / F12 (1:1) medium containing 10% fetal bovine serum to readjust the cell density 1×10 5 After inoculation in 100mm cell culture dish, placed in 5% CO 2 cultured in a 37°C cell culture incubator. At the same time, some human amniotic epithelial cells were collected for ...

Embodiment 3

[0068] Example 3 Preparation of Human Amniotic Epithelial Cell Secreted Factors (3)

[0069] 1. With the approval of the Ethics Committee of our hospital and the informed consent of the research subjects, fresh amniotic tissue from full-term healthy puerperas was obtained under sterile conditions.

[0070] 2. Digest amnion tissue with 0.25% trypsin for 3 minutes to separate human amniotic epithelial cells, resuspend in DMEM / F12 (1:1) medium containing 10% fetal bovine serum, inoculate in 100mm cell culture dish and place at 5% CO 2 cultured in a 37°C cell culture incubator.

[0071] 3. When the cell confluence reaches 70%-80%, use 0.25% trypsin to digest the adherent cells for 2 minutes and resuspend in DMEM / F12 (1:1) medium containing 10% fetal bovine serum to readjust the cell density 1×10 5 After inoculation in 100mm cell culture dish, placed in 5% CO 2 cultured in a 37°C cell culture incubator. At the same time, some human amniotic epithelial cells were collected for ...

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Abstract

The invention relates to an application of a human amniotic epithelial cell secretory factor in preparation of a medicine for ovarian function restoration. The human amniotic epithelial cell secretoryfactor is concentrated and then is injected to a chemotherapically-damaged mouse through intraperitoneal injection; it is found that the secretory factor can significantly increase the number of follicles in a damaged ovary, improve expression of genes related to development of the ovary, and promote regeneration of blood vessels. It is proved that the human amniotic epithelial cell can be directly differentiated into granular cells participating in the ovarian function restoration process, and a paracrine pathway also contributes important function to the process. The human amniotic epithelial cell secretory factor can be used for preparing the medicine for ovarian function restoration.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of human amniotic epithelial cell secreted factors in the preparation of drugs for restoring ovarian function. Background technique [0002] Human Amniotic Epithelial Cells (hAECs) come from the "medical waste" placenta after childbirth. They are rich in sources, easy to obtain, non-invasive, less likely to cause ethical and legal disputes, and have low immunogenicity. Because of its unique biological characteristics, it opens up a new research path for the functional repair of damaged tissues and the development of regenerative medicine. More and more studies have shown that the transplantation of human amniotic epithelial cells has a good therapeutic effect on various tissue damage and disease models. [0003] "Soochow University", 2013, 29 (5), published the paper "Research on Transplantation Immunity of Human Amniotic Epithelial Cells Transplantation in T...

Claims

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Application Information

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IPC IPC(8): A61K35/50A61P15/08C12N5/073C12P21/00
CPCA61K35/50C12N5/0605C12P21/00C12N2509/00
Inventor 赖东梅张秋婉郭英孙俊艳卜时夏王茜
Owner THE INT PEACE MATERNITY & CHILD HEALTH HOSPITAL OF CHINA WELFARE INST
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